NSA2, a novel nucleolus protein regulates cell proliferation and cell cycle

Journal Article · Fri Jan 01 04:00:00 EST 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.11.114· OSTI ID:22199979

Zhang, Heyu ^[1]; Ma, Xi ^[1]; Shi, Taiping ^[2]; Song, Quansheng ^[1]; Zhao, Hongshan ^[1]; Ma, Dalong ^[1]

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1. Department of Immunology, School of Basic Medical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191 (China)
2. Chinese National Human Genome Center, Beijing. 3-707 North YongChang Road BDA, Beijing 100176 (China)

NSA2 (Nop seven-associated 2) was previously identified in a high throughput screen of novel human genes associated with cell proliferation, and the NSA2 protein is evolutionarily conserved across different species. In this study, we revealed that NSA2 is broadly expressed in human tissues and cultured cell lines, and located in the nucleolus of the cell. Both of the putative nuclear localization signals (NLSs) of NSA2, also overlapped with nucleolar localization signals (NoLSs), are capable of directing nucleolar accumulation. Moreover, over-expression of the NSA2 protein promoted cell growth in different cell lines and regulated the G1/S transition in the cell cycle. SiRNA silencing of the NSA2 transcript attenuated the cell growth and dramatically blocked the cell cycle in G1/S transition. Our results demonstrated that NSA2 is a nucleolar protein involved in cell proliferation and cell cycle regulation.

• OSTI ID: 22199979
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 391; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL GROWTH
ANIMAL TISSUES
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
GENE REGULATION
PROTEINS
SIGNALS