skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards 'universal-donor' red blood cells

Abstract

Bombay phenotype is one of the rare phenotypes in the ABO blood group system that fails to express ABH antigens on red blood cells. Nonsense or missense mutations in fucosyltransfrase1 (FUT1) and fucosyltransfrase2 (FUT2) genes are known to create this phenotype. This blood group is compatible with all other blood groups as a donor, as it does not express the H antigen on the red blood cells. In this study, we describe the establishment of human induced pluripotent stem cells (iPSCs) from the dermal fibroblasts of a Bombay blood-type individual by the ectopic expression of established transcription factors Klf4, Oct4, Sox2, and c-Myc. Sequence analyses of fibroblasts and iPSCs revealed a nonsense mutation 826C to T (276 Gln to Ter) in the FUT1 gene and a missense mutation 739G to A (247 Gly to Ser) in the FUT2 gene in the Bombay phenotype under study. The established iPSCs resemble human embryonic stem cells in morphology, passaging, surface and pluripotency markers, normal karyotype, gene expression, DNA methylation of critical pluripotency genes, and in-vitro differentiation. The directed differentiation of the iPSCs into hematopoietic lineage cells displayed increased expression of the hematopoietic lineage markers such as CD34, CD133, RUNX1, KDR, {alpha}-globulin, and {gamma}-globulin.more » Such specific stem cells provide an unprecedented opportunity to produce a universal blood group donor, in-vitro, thus enabling cellular replacement therapies, once the safety issue is resolved.« less

Authors:
;  [1]; ;  [2];  [1];  [1];  [1];  [2];  [1]
  1. Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, P.O. Box 19395-4644, ACECR, Tehran (Iran, Islamic Republic of)
  2. Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran (Iran, Islamic Republic of)
Publication Date:
OSTI Identifier:
22199970
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 391; Journal Issue: 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; BLOOD CELLS; BLOOD GROUPS; DNA; FIBROBLASTS; GENES; GLOBULINS; IN VITRO; KARYOTYPE; METHYLATION; MUTATIONS; PHENOTYPE; STEM CELLS; THERAPY; TRANSCRIPTION FACTORS

Citation Formats

Seifinejad, Ali, Taei, Adeleh, Totonchi, Mehdi, Vazirinasab, Hamed, Hassani, Seideh Nafiseh, Aghdami, Nasser, Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Shahbazi, Ebrahim, Yazdi, Reza Salman, Salekdeh, Ghasem Hosseini, E-mail: Salekdeh@royaninstitute.org, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Baharvand, Hossein, Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, and Department of Developmental Biology, University of Science and Culture, ACECR, Tehran. Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards 'universal-donor' red blood cells. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2009.11.058.
Seifinejad, Ali, Taei, Adeleh, Totonchi, Mehdi, Vazirinasab, Hamed, Hassani, Seideh Nafiseh, Aghdami, Nasser, Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Shahbazi, Ebrahim, Yazdi, Reza Salman, Salekdeh, Ghasem Hosseini, E-mail: Salekdeh@royaninstitute.org, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Baharvand, Hossein, Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, & Department of Developmental Biology, University of Science and Culture, ACECR, Tehran. Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards 'universal-donor' red blood cells. United States. doi:10.1016/J.BBRC.2009.11.058.
Seifinejad, Ali, Taei, Adeleh, Totonchi, Mehdi, Vazirinasab, Hamed, Hassani, Seideh Nafiseh, Aghdami, Nasser, Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Shahbazi, Ebrahim, Yazdi, Reza Salman, Salekdeh, Ghasem Hosseini, E-mail: Salekdeh@royaninstitute.org, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Baharvand, Hossein, Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, and Department of Developmental Biology, University of Science and Culture, ACECR, Tehran. Fri . "Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards 'universal-donor' red blood cells". United States. doi:10.1016/J.BBRC.2009.11.058.
@article{osti_22199970,
title = {Generation of human induced pluripotent stem cells from a Bombay individual: Moving towards 'universal-donor' red blood cells},
author = {Seifinejad, Ali and Taei, Adeleh and Totonchi, Mehdi and Vazirinasab, Hamed and Hassani, Seideh Nafiseh and Aghdami, Nasser and Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran and Shahbazi, Ebrahim and Yazdi, Reza Salman and Salekdeh, Ghasem Hosseini, E-mail: Salekdeh@royaninstitute.org and Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj and Baharvand, Hossein and Department of Regenerative Biomedicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran and Department of Developmental Biology, University of Science and Culture, ACECR, Tehran},
abstractNote = {Bombay phenotype is one of the rare phenotypes in the ABO blood group system that fails to express ABH antigens on red blood cells. Nonsense or missense mutations in fucosyltransfrase1 (FUT1) and fucosyltransfrase2 (FUT2) genes are known to create this phenotype. This blood group is compatible with all other blood groups as a donor, as it does not express the H antigen on the red blood cells. In this study, we describe the establishment of human induced pluripotent stem cells (iPSCs) from the dermal fibroblasts of a Bombay blood-type individual by the ectopic expression of established transcription factors Klf4, Oct4, Sox2, and c-Myc. Sequence analyses of fibroblasts and iPSCs revealed a nonsense mutation 826C to T (276 Gln to Ter) in the FUT1 gene and a missense mutation 739G to A (247 Gly to Ser) in the FUT2 gene in the Bombay phenotype under study. The established iPSCs resemble human embryonic stem cells in morphology, passaging, surface and pluripotency markers, normal karyotype, gene expression, DNA methylation of critical pluripotency genes, and in-vitro differentiation. The directed differentiation of the iPSCs into hematopoietic lineage cells displayed increased expression of the hematopoietic lineage markers such as CD34, CD133, RUNX1, KDR, {alpha}-globulin, and {gamma}-globulin. Such specific stem cells provide an unprecedented opportunity to produce a universal blood group donor, in-vitro, thus enabling cellular replacement therapies, once the safety issue is resolved.},
doi = {10.1016/J.BBRC.2009.11.058},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 391,
place = {United States},
year = {2010},
month = {1}
}