CrxOS maintains the self-renewal capacity of murine embryonic stem cells

Journal Article · Fri Dec 25 04:00:00 EST 2009 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.09.118· OSTI ID:22199930

Saito, Ryota; Yamasaki, Tokiwa; Nagai, Yoko; Wu, Jinzhan ^[1]; Kajiho, Hiroaki ^[2]; Yokoi, Tadashi; Noda, Eiichiro ^[1]; Nishina, Sachiko ^[3]; Niwa, Hitoshi ^[4]; Azuma, Noriyuki ^[3]; Katada, Toshiaki ^[2]; Nishina, Hiroshi ^[1]

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1. Department of Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510 (Japan)
2. Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033 (Japan)
3. Department of Ophthalmology, National Center for Child Health and Development, Tokyo 157-8535 (Japan)
4. Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Hyogo 650-0047 (Japan)

Embryonic stem (ES) cells maintain pluripotency by self-renewal. Several homeoproteins, including Oct3/4 and Nanog, are known to be key factors in maintaining the self-renewal capacity of ES cells. However, other genes required for the mechanisms underlying this process are still unclear. Here we report the identification by in silico analysis of a homeobox-containing gene, CrxOS, that is specifically expressed in murine ES cells and is essential for their self-renewal. ES cells mainly express the short isoform of endogenous CrxOS. Using a polyoma-based episomal expression system, we demonstrate that overexpression of the CrxOS short isoform is sufficient for maintaining the undifferentiated morphology of ES cells and stimulating their proliferation. Finally, using RNA interference, we show that CrxOS is essential for the self-renewal of ES cells, and provisionally identify foxD3 as a downstream target gene of CrxOS. To our knowledge, ours is the first delineation of the physiological role of CrxOS in ES cells.

• OSTI ID: 22199930
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 390; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CAPACITY
CELL PROLIFERATION
GENES
MORPHOLOGY
RNA
STEM CELLS