The transmembrane nucleoporin NDC1 is required for targeting of ALADIN to nuclear pore complexes

Journal Article · Fri Nov 06 00:00:00 EST 2009 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.08.096· OSTI ID:22199862

Yamazumi, Yusuke; Kamiya, Atsushi; Nishida, Ayumu; Nishihara, Ayako ^[1]; Iemura, Shun-ichiro; Natsume, Tohru ^[2]; Akiyama, Tetsu ^[1]

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1. Laboratory of Molecular and Genetic Information, Institute for Molecular and Cellular Bioscience, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032 (Japan)
2. Biological Information Research Center (AIST), Japan Biological Information Research Center (JBIC), Aomi 2-41-6, Koutou-ku, Tokyo 135-0064 (Japan)

NDC1 is a transmembrane nucleoporin that is required for NPC assembly and nucleocytoplasmic transport. We show here that NDC1 directly interacts with the nucleoporin ALADIN, mutations of which are responsible for triple-A syndrome, and that this interaction is required for targeting of ALADIN to nuclear pore complexes (NPCs). Furthermore, we show that NDC1 is required for selective nuclear import. Our findings suggest that NDC1-mediated localization of ALADIN to NPCs is essential for selective nuclear protein import, and that abrogation of the interaction between ALADIN and NDC1 may be important for the development of triple-A syndrome.

• OSTI ID: 22199862
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 389, Issue 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL DIVISION
CELL PROLIFERATION
GENE REGULATION
MUTATIONS
PROTEINS