Down-regulation of lipoprotein lipase increases glucose uptake in L6 muscle cells
Journal Article
·
· Biochemical and Biophysical Research Communications
- Department of Chemistry and Biochemistry, California State University Northridge, Northridge, CA 91330-8262 (United States)
Thiazolidinediones (TZDs) are synthetic hypoglycemic agents used to treat type 2 diabetes. TZDs target the peroxisome proliferator activated receptor-gamma (PPAR-{gamma}) and improve systemic insulin sensitivity. The contributions of specific tissues to TZD action, or the downstream effects of PPAR-{gamma} activation, are not very clear. We have used a rat skeletal muscle cell line (L6 cells) to demonstrate that TZDs directly target PPAR-{gamma} in muscle cells. TZD treatment resulted in a significant repression of lipoprotein lipase (LPL) expression in L6 cells. This repression correlated with an increase in glucose uptake. Down-regulation of LPL message and protein levels using siRNA resulted in a similar increase in insulin-dependent glucose uptake. Thus, LPL down-regulation improved insulin sensitivity independent of TZDs. This finding provides a novel method for the management of insulin resistance.
- OSTI ID:
- 22199858
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 389; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
Similar Records
Bixin regulates mRNA expression involved in adipogenesis and enhances insulin sensitivity in 3T3-L1 adipocytes through PPAR{gamma} activation
Sp1 mediates repression of the resistin gene by PPAR{gamma} agonists in 3T3-L1 adipocytes
Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed
Journal Article
·
Thu Dec 24 23:00:00 EST 2009
· Biochemical and Biophysical Research Communications
·
OSTI ID:22199947
Sp1 mediates repression of the resistin gene by PPAR{gamma} agonists in 3T3-L1 adipocytes
Journal Article
·
Fri Sep 15 00:00:00 EDT 2006
· Biochemical and Biophysical Research Communications
·
OSTI ID:20854465
Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed
Journal Article
·
Fri Aug 24 00:00:00 EDT 2012
· Biochemical and Biophysical Research Communications
·
OSTI ID:22210196