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Title: Identification of a common microdeletion cluster in 7q21.3 subband among patients with myeloid leukemia and myelodysplastic syndrome

Abstract

Monosomy 7 and interstitial deletions in the long arm of chromosome 7 (-7/7q-) is a common nonrandom chromosomal abnormality found frequently in myeloid disorders including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and juvenile myelomonocytic leukemia (JMML). Using a short probe-based microarray comparative genomic hybridization (mCGH) technology, we identified a common microdeletion cluster in 7q21.3 subband, which is adjacent to 'hot deletion region' thus far identified by conventional methods. This common microdeletion cluster contains three poorly characterized genes; Samd9, Samd9L, and a putative gene LOC253012, which we named Miki. Gene copy number assessment of three genes by real-time PCR revealed heterozygous deletion of these three genes in adult patients with AML and MDS at high frequency, in addition to JMML patients. Miki locates to mitotic spindles and centrosomes and downregulation of Miki by RNA interference induced abnormalities in mitosis and nuclear morphology, similar to myelodysplasia. In addition, a recent report indicated Samd9 as a tumor suppressor. These findings indicate the usefulness of the short probe-based CGH to detect microdeletions. The three genes located to 7q21.3 would be candidates for myeloid tumor-suppressor genes on 7q.

Authors:
; ; ; ; ;  [1];  [1]
  1. Department of Molecular Oncology and Leukemia Program Project, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553 (Japan)
Publication Date:
OSTI Identifier:
22199707
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 383; Journal Issue: 2; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHROMOSOMES; GENES; MITOSIS; MORPHOLOGY; MYELOID LEUKEMIA; PATIENTS; POLYMERASE CHAIN REACTION; RNA

Citation Formats

Asou, Hiroya, Matsui, Hirotaka, Ozaki, Yuko, Nagamachi, Akiko, Nakamura, Megumi, Aki, Daisuke, and Inaba, Toshiya, E-mail: tinaba@hiroshima-u.ac.jp. Identification of a common microdeletion cluster in 7q21.3 subband among patients with myeloid leukemia and myelodysplastic syndrome. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.04.004.
Asou, Hiroya, Matsui, Hirotaka, Ozaki, Yuko, Nagamachi, Akiko, Nakamura, Megumi, Aki, Daisuke, & Inaba, Toshiya, E-mail: tinaba@hiroshima-u.ac.jp. Identification of a common microdeletion cluster in 7q21.3 subband among patients with myeloid leukemia and myelodysplastic syndrome. United States. doi:10.1016/J.BBRC.2009.04.004.
Asou, Hiroya, Matsui, Hirotaka, Ozaki, Yuko, Nagamachi, Akiko, Nakamura, Megumi, Aki, Daisuke, and Inaba, Toshiya, E-mail: tinaba@hiroshima-u.ac.jp. 2009. "Identification of a common microdeletion cluster in 7q21.3 subband among patients with myeloid leukemia and myelodysplastic syndrome". United States. doi:10.1016/J.BBRC.2009.04.004.
@article{osti_22199707,
title = {Identification of a common microdeletion cluster in 7q21.3 subband among patients with myeloid leukemia and myelodysplastic syndrome},
author = {Asou, Hiroya and Matsui, Hirotaka and Ozaki, Yuko and Nagamachi, Akiko and Nakamura, Megumi and Aki, Daisuke and Inaba, Toshiya, E-mail: tinaba@hiroshima-u.ac.jp},
abstractNote = {Monosomy 7 and interstitial deletions in the long arm of chromosome 7 (-7/7q-) is a common nonrandom chromosomal abnormality found frequently in myeloid disorders including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and juvenile myelomonocytic leukemia (JMML). Using a short probe-based microarray comparative genomic hybridization (mCGH) technology, we identified a common microdeletion cluster in 7q21.3 subband, which is adjacent to 'hot deletion region' thus far identified by conventional methods. This common microdeletion cluster contains three poorly characterized genes; Samd9, Samd9L, and a putative gene LOC253012, which we named Miki. Gene copy number assessment of three genes by real-time PCR revealed heterozygous deletion of these three genes in adult patients with AML and MDS at high frequency, in addition to JMML patients. Miki locates to mitotic spindles and centrosomes and downregulation of Miki by RNA interference induced abnormalities in mitosis and nuclear morphology, similar to myelodysplasia. In addition, a recent report indicated Samd9 as a tumor suppressor. These findings indicate the usefulness of the short probe-based CGH to detect microdeletions. The three genes located to 7q21.3 would be candidates for myeloid tumor-suppressor genes on 7q.},
doi = {10.1016/J.BBRC.2009.04.004},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 383,
place = {United States},
year = 2009,
month = 5
}
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