Characterization of cell lines stably transfected with rubella virus replicons

Journal Article · Fri Jul 20 04:00:00 EDT 2012 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2012.04.003· OSTI ID:22149281

Tzeng, Wen-Pin; Xu, Jie ^[1]

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1. Department of Biology, Georgia State University, P.O. Box 4010, Atlanta GA 30302-4010 (United States)

Rubella virus (RUBV) replicons expressing a drug resistance gene and a gene of interest were used to select cell lines uniformly harboring the replicon. Replicons expressing GFP and a virus capsid protein GFP fusion (C-GFP) were compared. Vero or BHK cells transfected with either replicon survived drug selection and grew into a monolayer. However, survival was {approx}9-fold greater following transfection with the C-GFP-replicon than with the GFP-expressing replicon and while the C-GFP-replicon cells grew similarly to non-transfected cells, the GFP-replicon cells grew slower. Neither was due to the ability of the CP to enhance RNA synthesis but survival during drug selection was correlated with the ability of CP to inhibit apoptosis. Additionally, C-GFP-replicon cells were not cured of the replicon in the absence of drug selection. Interferon-alpha suppressed replicon RNA and protein synthesis, but did not cure the cells, explaining in part the ability of RUBV to establish persistent infections.

• OSTI ID: 22149281
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 429; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
DRUGS
INTERFERON
MEASLES VIRUS
REPLICONS
RNA
SYNTHESIS