NRIP enhances HPV gene expression via interaction with either GR or E2
- Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan (China)
- Department of Microbiology and Immunology, National Chiayi University, Chiayi 600-04, Taiwan (China)
- Department of Ophthalmology, Mackay Memorial Hospital, Taipei 104, Taiwan (China)
We previously identified a gene, nuclear receptor-interaction protein (NRIP), which functions as a transcription cofactor in glucocorticoid receptor (GR) and human papillomavirus E2 (HPV E2)-driven gene expression. Here, we comprehensively evaluated the role of NRIP in HPV-16 gene expression. NRIP acts as a transcription cofactor to enhance GR-regulated HPV-16 gene expression in the presence of hormone. NRIP also can form complex with E2 that caused NRIP-induced HPV gene expression via E2-binding sites in a hormone-independent manner. Furthermore, NRIP can associate with GR and E2 to form tri-protein complex to activate HPV gene expression via GRE, not the E2-binding site, in a hormone-dependent manner. These results indicate that NRIP and GR are viral E2-binding proteins and that NRIP regulates HPV gene expression via GRE and/or E2 binding site in the HPV promoter in a hormone-dependent or independent manner, respectively.
- OSTI ID:
- 22149257
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 1 Vol. 423; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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