Confocal scanning microscopy under ultrashort pulse illumination
Conference
·
OSTI ID:220819
- Univ. of Sydney, New South Wales (Australia). Dept. of Physical Optics
The authors, in this paper, investigate resolution in various confocal imaging methods based on the use of an ultrashort pulsed laser beam. For bright-field confocal microscopy, time-resolved and time averaged imaging methods are considered, while resolution in two-photon and single-photon confocal fluorescence microscopy is examined. It is shown that the time-resolved approach can give a three-dimensional image of a thick object with axial resolution approximately 30% higher than the limiting value under CW laser illumination of the same wavelength as the pulsed beam, while the transverse resolution is slightly improved.
- OSTI ID:
- 220819
- Report Number(s):
- CONF-950793--
- Country of Publication:
- United States
- Language:
- English
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