3D surface topography study of the biofunctionalized nanocrystalline Ti-6Zr-4Nb/Ca-P

Journal Article · Wed Aug 15 00:00:00 EDT 2012 · Materials Characterization

DOI:https://doi.org/10.1016/J.MATCHAR.2012.05.006· OSTI ID:22066481

Jakubowicz, J ^[1]; Adamek, G ^[1]; Jurczyk, M U ^[2]; Jurczyk, M ^[1]

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1. Poznan University of Technology, Institute of Materials Science and Engineering, M. Sklodowska-Curie 5 Sq., 60-965 Poznan (Poland)
2. Poznan University of Medical Sciences, Mothers and Childs Health Division, Polna 33, 60-535 Poznan (Poland)

In this work surface of the sintered Ti-6Zr-4Nb nanocrystalline alloy was electrochemically biofunctionalized. The porous surface was produced by anodic oxidation in 1 M H{sub 3}PO{sub 4} + 2%HF electrolyte at 10 V for 30 min. Next the calcium-phosphate (Ca-P) layer was deposited, onto the formed porous surface, using cathodic potential - 5 V kept for 60 min in 0.042 M Ca(NO{sub 3}){sub 2} + 0.025 M (NH{sub 4}){sub 2}HPO{sub 4} + 0.1 M HCl electrolyte. The deposited Ca-P layer anchored in the pores. The biofunctionalized surface was studied by XRD, SEM and EDS. In vitro tests culture of normal human osteoblast (NHOst) cells showed very good cells proliferation, colonization and multilayering. Using optical profiler, roughness and hybrid 3D surface topography parameters were estimated. Correlation between surface composition, morphology, roughness and biocompatibility results was done. It has been shown by us that surface with appropriate chemical composition and topography, after combined electrochemical anodic and cathodic surface treatment, supports osteoblast adhesion and proliferation. 3D topography measurements using optical profiler play a key role in the biomaterials surface analysis. - Highlights: Black-Right-Pointing-Pointer Nanocrystalline Ti-6Zr-4Nb/Ca-P material was produced for hard tissue implant applications. Black-Right-Pointing-Pointer Calcium-phosphate results in surface biofunctionalization. Black-Right-Pointing-Pointer The biofunctionalized surface shows good in-vitro behavior.

• OSTI ID: 22066481
• Journal Information: Materials Characterization, Vol. 70, Issue Complete; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 1044-5803
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
77 NANOSCIENCE AND NANOTECHNOLOGY
60 APPLIED LIFE SCIENCES
AMMONIUM PHOSPHATES
CALCIUM NITRATES
CALCIUM PHOSPHATES
CHEMICAL COMPOSITION
CONNECTIVE TISSUE CELLS
CRYSTALS
ELECTROCHEMISTRY
IMPLANTS
IN VITRO
NANOSTRUCTURES
PHOSPHORIC ACID
POROUS MATERIALS
ROUGHNESS
SCANNING ELECTRON MICROSCOPY
SURFACE TREATMENTS
SURFACES
TITANIUM BASE ALLOYS
TOPOGRAPHY
X-RAY DIFFRACTION
ZIRCONIUM ALLOYS