Absorbance and fluorometric sensing with capillary wells microplates
- Laboratory for Optics, Acoustics, and Mechanics, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria 3800 (Australia)
- Cardiovascular Biomarkers Laboratory, Cardiovascular Research Institute, 30 Medical Drive, Singapore 117609 (Singapore)
Detection and readout from small volume assays in microplates are a challenge. The capillary wells microplate approach [Ng et al., Appl. Phys. Lett. 93, 174105 (2008)] offers strong advantages in small liquid volume management. An adapted design is described and shown here to be able to detect, in a nonimaging manner, fluorescence and absorbance assays minus the error often associated with meniscus forming at the air-liquid interface. The presence of bubbles in liquid samples residing in microplate wells can cause inaccuracies. Pipetting errors, if not adequately managed, can result in misleading data and wrong interpretations of assay results; particularly in the context of high throughput screening. We show that the adapted design is also able to detect for bubbles and pipetting errors during actual assay runs to ensure accuracy in screening.
- OSTI ID:
- 22062222
- Journal Information:
- Review of Scientific Instruments, Vol. 81, Issue 12; Other Information: (c) 2010 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0034-6748
- Country of Publication:
- United States
- Language:
- English
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