Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast

Carvalho Borges, Pâmela C.; Bouabboune, Chaïnez; Escandell, Jose Miguel; Matmati, Samah; Coulon, Stéphane; Ferreira, Miguel Godinho

doi:10.1093/nar/gkad1036

Title: Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast

Journal Article · Sat Nov 11 00:00:00 EST 2023 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkad1036· OSTI ID:2205520

; Bouabboune, Chaïnez;

; Matmati, Samah;

;

Abstract Telomeres are nucleoprotein complexes that protect the chromosome-ends from eliciting DNA repair while ensuring their complete duplication. Pot1 is a subunit of telomere capping complex that binds to the G-rich overhang and inhibits the activation of DNA damage checkpoints. In this study, we explore new functions of fission yeast Pot1 by using a pot1-1 temperature sensitive mutant. We show that pot1 inactivation impairs telomere DNA replication resulting in the accumulation of ssDNA leading to the complete loss of telomeric DNA. Recruitment of Stn1 to telomeres, an auxiliary factor of DNA lagging strand synthesis, is reduced in pot1-1 mutants and overexpression of Stn1 rescues loss of telomeres and cell viability at restrictive temperature. We propose that Pot1 plays a crucial function in telomere DNA replication by recruiting Stn1-Ten1 and Polα-primase complex to telomeres via Tpz1, thus promoting lagging-strand DNA synthesis at stalled replication forks.

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Sponsoring Organization:: USDOE Office of Nuclear Energy (NE), Nuclear Fuel Cycle and Supply Chain

Grant/Contract Number:: PTDC/BEX-BCM/5179/14; Action 2 - 2019

OSTI ID:: 2205520

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Vol. 51 Journal Issue: 22; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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