Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study

Journal Article · Wed Sep 15 00:00:00 EDT 2010 · Crystallography Reports

DOI:https://doi.org/10.1134/S1063774510050159· OSTI ID:22054091

Serov, A E; Skirgello, O E ^[1]; Timofeev, V I; Samygina, V R ^[2]; Popov, V O ^[3]; Tishkov, V I ^[1]; Kuranova, I P ^[2]

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1. Moscow State University, Faculty of Chemistry (Russian Federation)
2. Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)
3. Russian Academy of Sciences, Bach Institute of Biochemistry (Russian Federation)

Crystals of high-purity recombinant NAD{sup +}-dependent formate dehydrogenase from the higher plant Arabidopsis thaliana (AraFDH) were grown in microgravity in the Modul'-1 protein crystallization apparatus on the International Space Station. The space-grown crystals have larger sizes than those grown on Earth. X-ray diffraction data suitable for determining the three-dimensional structure were collected from the space-grown crystals to a resolution of 1.22 A using an X-ray synchrotron source. The crystals belong to sp. gr. P4{sub 3}2{sub 1}2; the unit-cell parameters are a = b = 107.865 A, c = 71.180 A, {alpha} = {beta} = {gamma} = 90 deg.

• OSTI ID: 22054091
• Journal Information: Crystallography Reports, Vol. 55, Issue 5; Other Information: Copyright (c) 2010 Pleiades Publishing, Ltd.; Country of input: International Atomic Energy Agency (IAEA); ISSN 1063-7745
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
ARABIDOPSIS
CRYSTAL GROWTH
CRYSTALLIZATION
CRYSTALS
FORMATES
IMPURITIES
RESOLUTION
TETRAGONAL LATTICES
X-RAY DIFFRACTION