Field-amplified sample stacking and focusing in nanofluidic channels
- Department of Mechanical Engineering, University of California, Santa Barbara, California 93106 (United States)
- Franklin W. Olin College of Engineering, Needham, Massachusetts 02492 (United States)
Nanofluidic technology is gaining popularity for bioanalytical applications due to advances in both nanofabrication and design. One major obstacle in the widespread adoption of such technology for bioanalytical systems is efficient detection of samples due to the inherently low analyte concentrations present in such systems. This problem is exacerbated by the push for electronic detection, which requires an even higher sensor-local sample concentration than optical detection. This paper explores one of the most common preconcentration techniques, field-amplified sample stacking, in nanofluidic systems in efforts to alleviate this obstacle. Holding the ratio of background electrolyte concentrations constant, the parameters of channel height, strength of electric field, and concentration are varied. Although in micron scale systems, these parameters have little or no effect on the final concentration enhancement achieved, nanofluidic experiments show strong dependencies on each of these parameters. Further, nanofluidic systems demonstrate an increased concentration enhancement over what is predicted and realized in microscale counterparts. Accordingly, a depth-averaged theoretical model is developed that explains these observations and furthermore predicts a novel focusing mechanism that can explain the increased concentration enhancement achieved. Specifically, when the electric double layer is sufficient in size relative to the channel height, negatively charged analyte ions are repelled from negatively charged walls, and thus prefer to inhabit the centerline of the channels. The resulting induced pressure gradients formed due to the high and low electrical conductivity fluids in the channel force the ions to move at a slower velocity in the low-conductivity region, and a faster velocity in the high-conductivity region, leading to focusing. A simple single-channel model is capable of predicting key experimental observations, while a model that incorporates the details of the fluid inlet and outlet ports allows for more detailed comparisons between model and experiment.
- OSTI ID:
- 22038533
- Journal Information:
- Physics of Fluids (1994), Vol. 22, Issue 11; Other Information: (c) 2010 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 1070-6631
- Country of Publication:
- United States
- Language:
- English
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