Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues

Cao, Viet Dang; Luo, Guangbin; Korynta, Shelby; Liu, Hui; Liang, Yuanxue; Shanklin, John; Altpeter, Fredy

doi:10.1186/s13068-023-02393-1

Title: Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues

Journal Article · Sat Oct 14 00:00:00 EDT 2023 · Biotechnology for Biofuels and Bioproducts

DOI:https://doi.org/10.1186/s13068-023-02393-1· OSTI ID:2202296

Cao, Viet Dang; Luo, Guangbin; Korynta, Shelby; Liu, Hui; Liang, Yuanxue; Shanklin, John; Altpeter, Fredy

Abstract Background Metabolic engineering for hyperaccumulation of lipids in vegetative tissues is a novel strategy for enhancing energy density and biofuel production from biomass crops. Energycane is a prime feedstock for this approach due to its high biomass production and resilience under marginal conditions. DIACYLGLYCEROL ACYLTRANSFERASE ( DGAT ) catalyzes the last and only committed step in the biosynthesis of triacylglycerol (TAG) and can be a rate-limiting enzyme for the production of TAG. Results In this study, we explored the effect of intron-mediated enhancement (IME) on the expression of DGAT 1 and resulting accumulation of TAG and total fatty acid (TFA) in leaf and stem tissues of energycane. To maximize lipid accumulation these evaluations were carried out by co-expressing the lipogenic transcription factor WRINKLED 1 ( WRI 1) and the TAG protect factor oleosin ( OLE 1). Including an intron in the codon-optimized TmDGAT 1 elevated the accumulation of its transcript in leaves by seven times on average based on 5 transgenic lines for each construct. Plants with WRI 1 (W), DGAT 1 with intron (Di), and OLE 1 (O) expression (WDiO) accumulated TAG up to a 3.85% of leaf dry weight (DW), a 192-fold increase compared to non-modified energycane (WT) and a 3.8-fold increase compared to the highest accumulation under the intron-less gene combination (WDO). This corresponded to TFA accumulation of up to 8.4% of leaf dry weight, a 2.8-fold or 6.1-fold increase compared to WDO or WT, respectively. Co-expression of WDiO resulted in stem accumulations of TAG up to 1.14% of DW or TFA up to 2.08% of DW that exceeded WT by 57-fold or 12-fold and WDO more than twofold, respectively. Constitutive expression of these lipogenic “push pull and protect” factors correlated with biomass reduction. Conclusions Intron-mediated enhancement (IME) of the expression of DGAT resulted in a step change in lipid accumulation of energycane and confirmed that under our experimental conditions it is rate limiting for lipid accumulation. IME should be applied to other lipogenic factors and metabolic engineering strategies. The findings from this study may be valuable in developing a high biomass feedstock for commercial production of lipids and advanced biofuels. Graphical abstract

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA National Institute of Food and Agriculture

Grant/Contract Number:: SC0018254; SC0012704; SC0018420

OSTI ID:: 2202296

Alternate ID(s):: OSTI ID: 2222378

Report Number(s):: BNL-225005-2023-JAAM; 153; PII: 2393

Journal Information:: Biotechnology for Biofuels and Bioproducts, Journal Name: Biotechnology for Biofuels and Bioproducts Vol. 16 Journal Issue: 1; ISSN 2731-3654

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Energycane
WRINKLED1
DIACYLGLYCEROL ACYLTRANSFERASE1
OLEOSIN1
Triacylglycerol
Metabolic engineering
Lipids
Biofuel
Bioenergy
Intron-mediated enhancement
Transgene expression

Title: Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues

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