Diadenosine tetraphosphate (AP{sub 4}A) levels in muscle tissue as a potential biomarker of PCB and TCDD exposure

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:218433

Nordone, A ^[1]; Grant, T; Saranko, C; Pivorun, E ^[2]

---

1. Consultox Ltd., Damariscotta, ME (United States)
2. Clemson Univ., SC (United States). Dept. of Biological Sciences

During the past two decades a group of purine nucleotides, the diadenosine polyphosphates, have gained increasing attention. One of these compounds, diadenosine tetraphosphate (AP{sub 4}A), has been demonstrated to modulate a variety of cellular events, including intracellular signaling during adaptive responses to cellular stress. The authors have recently demonstrated that changes in AP{sub 4}A levels in venous and arterial blood are related to periods of major trauma during corrective spinal surgery. Using Deermice (Peromyscus maniculatus) they investigated whether changes in the concentration of AP{sub 4}A in muscle tissue could be used as a biomarker of chemically induced stress. Using intraperitoneal administration, animals were exposed to either a mixture of PCB congeners (Aroclor 1254), a coplanar PCB (PCB 169), a noncoplanar PCB (PCB 153) or TCDD for a period of 11 days. The coplanar PCB and TCDD decreased AP{sub 4}A concentrations in dose-dependent manner, whereas the noncoplanar PCB increased concentrations in a dose-dependent manner. Aroclor 1254 reduced AP{sub 4}A concentration to a similar level at all doses. These results indicate that PCBs and TCDD modulate AP{sub 4}A concentrations in muscle tissue in a manner that is both dose and structure dependent. It is possible that AP{sub 4}A could be used as a marker of the level of exposure to PCBs and dioxin and as an indicator of the congeners involved.

• OSTI ID: 218433
• Report Number(s): CONF-9511137--; ISBN 1-880611-03-1
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL MARKERS
BIOLOGICAL STRESS
BIOSYNTHESIS
DIOXIN
DOSE-RESPONSE RELATIONSHIPS
EVALUATION
MICE
NUCLEOTIDES
POLYCHLORINATED BIPHENYLS
TOXICITY