Percutaneous Irreversible Electroporation Lung Ablation: Preliminary Results in a Porcine Model

Journal Article · Wed Dec 14 23:00:00 EST 2011 · Cardiovascular and Interventional Radiology

DOI:https://doi.org/10.1007/S00270-011-0143-9· OSTI ID:21608662

Deodhar, Ajita ^[1]; Monette, Sebastien ^[2]; Single, Gordon W; Hamilton, William C ^[3]; Thornton, Raymond H; Sofocleous, Constantinos T; Maybody, Majid

---

1. Memorial Sloan Kettering Cancer Center, Interventional Radiology and Image-Guided Therapies (United States)
2. Memorial Sloan Kettering Cancer Center, Laboratory of Comparative Pathology (United States)
3. Angiodynamics, Inc (United States)

Objective: Irreversible electroporation (IRE) uses direct electrical pulses to create permanent 'pores' in cell membranes to cause cell death. In contrast to conventional modalities, IRE has a nonthermal mechanism of action. Our objective was to study the histopathological and imaging features of IRE in normal swine lung. Materials and Methods: Eleven female swine were studied for hyperacute (8 h), acute (24 h), subacute (96 h), and chronic (3 week) effects of IRE ablation in lung. Paired unipolar IRE applicators were placed under computed tomography (CT) guidance. Some applicators were deliberately positioned near bronchovascular structures. IRE pulse delivery was synchronized with the cardiac rhythm only when ablation was performed within 2 cm of the heart. Contrast-enhanced CT scan was performed immediately before and after IRE and at 1 and 3 weeks after IRE ablation. Representative tissue was stained with hematoxylin and eosin for histopathology. Results: Twenty-five ablations were created: ten hyperacute, four acute, and three subacute ablations showed alveolar edema and necrosis with necrosis of bronchial, bronchiolar, and vascular epithelium. Bronchovascular architecture was maintained. Chronic ablations showed bronchiolitis obliterans and alveolar interstitial fibrosis. Immediate post-procedure CT images showed linear or patchy density along the applicator tract. At 1 week, there was consolidation that resolved partially or completely by 3 weeks. Pneumothorax requiring chest tube developed in two animals; no significant cardiac arrhythmias were noted. Conclusion: Our preliminary porcine study demonstrates the nonthermal and extracellular matrix sparing mechanism of action of IRE. IRE is a potential alternative to thermal ablative modalities.

• OSTI ID: 21608662
• Journal Information: Cardiovascular and Interventional Radiology, Journal Name: Cardiovascular and Interventional Radiology Journal Issue: 6 Vol. 34; ISSN 0174-1551; ISSN CAIRDG
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ABLATION
ANIMAL TISSUES
ANIMALS
APOPTOSIS
AROMATICS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CAT SCANNING
CELL CONSTITUENTS
CELL MEMBRANES
CHEST
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
DOMESTIC ANIMALS
DYES
EDEMA
EOSIN
EPITHELIUM
FEMALES
FIBROSIS
HEART
HEMATOXYLIN
HETEROCYCLIC COMPOUNDS
HETEROCYCLIC OXYGEN COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
INDICATORS
LUNGS
MAMMALS
MATERIALS
MEMBRANES
NECROSIS
ORGANIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHENOLS
POLYPHENOLS
PYRANS
RESPIRATORY SYSTEM
SWINE
SYMPTOMS
TOMOGRAPHY
VERTEBRATES