The potential effect of metallothionein 2A - 5 A/G single nucleotide polymorphism on blood cadmium, lead, zinc and copper levels

Journal Article · Sat Oct 01 00:00:00 EDT 2011 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2011.06.023· OSTI ID:21587850

Kayaalti, Zeliha; Aliyev, Vugar; Soeylemezoglu, Tuelin

Metallothioneins (MTs) are low molecular weight, cysteine-rich, metal-binding proteins. Because of their rich thiol groups, MTs bind to the biologically essential metals and perform these metals' homeostatic regulations; absorb the heavy metals and assist with their transportation and extraction. The aim of this study was to investigate the association between the metallothionein 2A (MT2A) core promoter region - 5 A/G single nucleotide polymorphism (SNP) and Cd, Pb, Zn and Cu levels in the blood samples. MT2A polymorphism was determined by the standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique using the 616 blood samples and the genotype frequencies were found as 86.6% homozygote typical (AA), 12.8% heterozygote (AG) and 0.6% homozygote atypical (GG). Metal levels were analyzed by dual atomic absorption spectrophotometer system and the average levels of Cd, Pb, Zn and Cu in the blood samples were 1.69 {+-} 1.57 ppb, 30.62 {+-} 14.13 ppb, 0.98 {+-} 0.49 ppm and 1.04 {+-} 0.45 ppm, respectively. As a result; highly statistically significant associations were detected between the - 5 A/G core promoter region SNP in the MT2A gene and Cd, Pb and Zn levels (p = 0.004, p = 0.012 and p = 0.002, respectively), but no association was found with Cu level (p = 0.595). Individuals with the GG genotype had statistically lower Zn level and higher Cd and Pb levels in the blood samples than individuals with AA and AG genotypes. This study suggests that having the GG genotype individuals may be more sensitive for the metal toxicity and they should be more careful about protecting their health against the toxic effects of the heavy metals. - Highlights: > MT2A -5A/G SNP has strong effect on the Cd, Pb and Zn levels in the blood. > MT2A GG individuals should be more careful for their health against metal toxicity. > This SNP might be considered as a biomarker for risk of disease related to metals.

• OSTI ID: 21587850
• Journal Information: Toxicology and Applied Pharmacology, Vol. 256, Issue 1; Other Information: DOI: 10.1016/j.taap.2011.06.023; PII: S0041-008X(11)00256-0; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABSORPTION
BIOLOGICAL MARKERS
BLOOD
CADMIUM
COPPER
CYSTEINE
DISEASES
EXTRACTION
GENES
GENOTYPE
HEAVY METALS
METALLOTHIONEIN
NUCLEOTIDES
POLYMERASE CHAIN REACTION
TOXICITY
ZINC
AMINO ACIDS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACIDS
ELEMENTS
GENE AMPLIFICATION
MATERIALS
METALLOPROTEINS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
SEPARATION PROCESSES
SORPTION
THIOLS
TRANSITION ELEMENTS