Sulforaphane prevents microcystin-LR-induced oxidative damage and apoptosis in BALB/c mice

Journal Article · Mon Aug 15 00:00:00 EDT 2011 · Toxicology and Applied Pharmacology

OSTI ID:21587810

Xiaoyun, Sun ^[1]; Lixin, Mi ^[2]; Jin, Liu ^[1]; Funglung, Chung ^[2]

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1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, CAS (China)
2. Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, D.C. 20057 (United States)

Microcystins (MCs), the products of blooming algae Microcystis, are waterborne environmental toxins that have been implicated in the development of liver cancer, necrosis, and even fatal intrahepatic bleeding. Alternative protective approaches in addition to complete removal of MCs in drinking water are urgently needed. In our previous work, we found that sulforaphane (SFN) protects against microcystin-LR (MC-LR)-induced cytotoxicity by activating the NF-E2-related factor 2 (Nrf2)-mediated defensive response in human hepatoma (HepG2) and NIH 3T3 cells. The purpose of this study was to investigate and confirm efficacy the SFN-induced multi-mechanistic defense system against MC-induced hepatotoxicity in an animal model. We report that SFN protected against MC-LR-induced liver damage and animal death at a nontoxic and physiologically relevant dose in BALB/c mice. The protection by SFN included activities of anti-cytochrome P450 induction, anti-oxidation, anti-inflammation, and anti-apoptosis. Our results suggest that SFN may protect mice against MC-induced hepatotoxicity. This raises the possibility of a similar protective effect in human populations, particularly in developing countries where freshwaters are polluted by blooming algae. - Graphical abstract: Display Omitted Research Highlights: > SFN protected against MC-LR-induced liver damage and animal death in BALB/c mice. > The dose of SFN is at a nontoxic and physiologically relevant dose. > The protection included activities of anti-oxidation, anti-inflammation, and anti-apoptosis. > SFN may protect mice against MC-induced hepatotoxicity.

• OSTI ID: 21587810
• Journal Information: Toxicology and Applied Pharmacology, Vol. 255, Issue 1; Other Information: DOI: 10.1016/j.taap.2011.05.011; PII: S0041-008X(11)00190-6; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALANINES
ALGAE
APOPTOSIS
DAMAGE
DEVELOPING COUNTRIES
DOSES
DRINKING WATER
GLUTATHIONE
HEPATOMAS
HUMAN POPULATIONS
INFLAMMATION
LIVER
MICE
NECROSIS
OXIDATION
PHOSPHATES
SAFETY
TOXICITY
TOXINS
AMINO ACIDS
ANIMALS
ANTIGENS
BODY
CARBOXYLIC ACIDS
CARCINOMAS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DISEASES
DRUGS
GLANDS
HAZARDOUS MATERIALS
HYDROGEN COMPOUNDS
MAMMALS
MATERIALS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDES
PHOSPHORUS COMPOUNDS
PLANTS
POLYPEPTIDES
POPULATIONS
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RESPONSE MODIFYING FACTORS
RODENTS
SYMPTOMS
TOXIC MATERIALS
VERTEBRATES
WATER