X-ray propagation microscopy of biological cells using waveguides as a quasipoint source

Journal Article · Tue Feb 15 00:00:00 EST 2011 · Physical Review. A

DOI:https://doi.org/10.1103/PHYSREVA.83.023804· OSTI ID:21537214

Giewekemeyer, K; Krueger, S P; Kalbfleisch, S; Bartels, M; Salditt, T ^[1]; Beta, C ^[2]

---

1. Institut fuer Roentgenphysik, Georg-August-Universitaet Goettingen, 37077 D-Goettingen (Germany)
2. Institut fuer Physik und Astronomie, Universitaet Potsdam, 14476 Potsdam/Golm, D-Germany (Germany)

We have used x-ray waveguides as highly confining optical elements for nanoscale imaging of unstained biological cells using the simple geometry of in-line holography. The well-known twin-image problem is effectively circumvented by a simple and fast iterative reconstruction. The algorithm which combines elements of the classical Gerchberg-Saxton scheme and the hybrid-input-output algorithm is optimized for phase-contrast samples, well-justified for imaging of cells at multi-keV photon energies. The experimental scheme allows for a quantitative phase reconstruction from a single holographic image without detailed knowledge of the complex illumination function incident on the sample, as demonstrated for freeze-dried cells of the eukaryotic amoeba Dictyostelium discoideum. The accessible resolution range is explored by simulations, indicating that resolutions on the order of 20 nm are within reach applying illumination times on the order of minutes at present synchrotron sources.

• OSTI ID: 21537214
• Journal Information: Physical Review. A, Vol. 83, Issue 2; Other Information: DOI: 10.1103/PhysRevA.83.023804; (c) 2011 American Institute of Physics; ISSN 1050-2947
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
77 NANOSCIENCE AND NANOTECHNOLOGY
ALGORITHMS
AMOEBA
HOLOGRAPHY
ILLUMINANCE
ITERATIVE METHODS
KEV RANGE
LYOPHILIZATION
MICROSCOPY
NANOSTRUCTURES
PHOTONS
RESOLUTION
SIMULATION
SYNCHROTRON RADIATION SOURCES
WAVEGUIDES
X RADIATION
ANIMALS
BOSONS
CALCULATION METHODS
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENERGY RANGE
INVERTEBRATES
IONIZING RADIATIONS
MASSLESS PARTICLES
MATHEMATICAL LOGIC
MICROORGANISMS
PROTOZOA
RADIATION SOURCES
RADIATIONS
SARCODINA