Local x-ray structure analysis of optically manipulated biological micro-objects

Journal Article · Mon Dec 13 00:00:00 EST 2010 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.3525941· OSTI ID:21518214

Cojoc, Dan; Ferrari, Enrico; Santucci, Silvia C ^[1]; Amenitsch, Heinz; Sartori, Barbara; Rappolt, Michael; Marmiroli, Benedetta ^[2]; Burghammer, Manfred; Riekel, Christian ^[3]

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1. Laboratorio TASC, Istituto Officina dei Materiali IOM-CNR, Trieste 34149 (Italy)
2. Institute for Biophysics and Nanosystems Research, Austrian Academy of Sciences, 8042 - Graz (Austria)
3. ID13 Microfocus Beamline, European Synchrotron Radiation Facility, 38043 - Grenoble (France)

X-ray diffraction using micro- and nanofocused beams is well suited for nanostructure analysis at different sites of a biological micro-object. To conduct in vitro studies without mechanical contact, we developed object manipulation by optical tweezers in a microfluidic cell. Here we report x-ray microdiffraction analysis of a micro-object optically trapped in three dimensions. We revealed the nanostructure of a single starch granule at different points and investigated local radiation damage induced by repeated x-ray exposures at the same position, demonstrating high stability and full control of the granule orientation by multiple optical traps.

• OSTI ID: 21518214
• Journal Information: Applied Physics Letters, Vol. 97, Issue 24; Other Information: DOI: 10.1063/1.3525941; (c) 2010 American Institute of Physics; ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
62 RADIOLOGY AND NUCLEAR MEDICINE
BEAMS
BIOLOGICAL RADIATION EFFECTS
IN VITRO
NANOSTRUCTURES
RADIATION PRESSURE
STABILITY
TRAPPING
X RADIATION
X-RAY DIFFRACTION
BIOLOGICAL EFFECTS
COHERENT SCATTERING
DIFFRACTION
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
RADIATION EFFECTS
RADIATIONS
SCATTERING