Fast Neutron Induced Autophagy Leads To Necrosis In Glioblastoma Multiforme Cells

Journal Article · Wed Jun 01 00:00:00 EDT 2011 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3586124· OSTI ID:21513382

Yasui, Linda; Gladden, Samantha ^[1]; Andorf, Christine ^[2]; Kroc, Thomas ^[3]

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1. Northern Illinois University, Department of Biological Sciences, DeKalb, IL 60115 (United States)
2. Northern Illinois Institute for Neutron Therapy, Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States)
3. Fermi National Accelerator Laboratory, Batavia, IL 60510 (United States)

Fast neutrons are highly effective at killing glioblastoma multiforme (GBM), U87 and U251 cells. The mode of cell death was investigated using transmission electron microscopy (TEM) to identify the fraction of irradiated U87 or U251 cells having morphological features of autophagy and/or necrosis. U87 or U251 cells were irradiated with 2 Gy fast neturons or 10 Gy {gamma} rays. A majority of U87 and U251 cells exhibit features of cell death with autophagy after irradiation with either 10 Gy {gamma} rays or 2 Gy fast neutrons. Very few {gamma} irradiated cells had features of necrosis (U87 or U251 cell samples processed for TEM 1 day after 10 Gy {gamma} irradiation). In contrast, a significant increase was observed in necrotic U87 and U251 cells irradiated with fast neutrons. These results show a greater percentage of cells exhibit morphological evidence of necrosis induced by a lower dose of fast neutron irradiation compared to {gamma} irradiation. Also, the evidence of necrosis in fast neutron irradiated U87 and U251 cells occurs in a background of autophagy. Since autophagy is observed before necrosis, autophagy may play a role in signaling programmed necrosis in fast neutron irradiated U87 and U251 cells.

• OSTI ID: 21513382
• Journal Information: AIP Conference Proceedings, Vol. 1336, Issue 1; Conference: CAARI 2010: 21. International Conference on the Application of Accelerators in Research and Industry, Fort Worth, TX (United States), 8-13 Aug 2010; Other Information: DOI: 10.1063/1.3586124; (c) 2011 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
62 RADIOLOGY AND NUCLEAR MEDICINE
APOPTOSIS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
FAST NEUTRONS
GAMMA RADIATION
GLIOMAS
IRRADIATION
NECROSIS
NUCLEAR MEDICINE
RADIATION DOSES
RADIOTHERAPY
TRANSMISSION ELECTRON MICROSCOPY
BARYONS
BIOLOGICAL EFFECTS
DISEASES
DOSES
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
ELEMENTARY PARTICLES
FERMIONS
HADRONS
IONIZING RADIATIONS
MEDICINE
MICROSCOPY
NEOPLASMS
NERVOUS SYSTEM DISEASES
NEUTRONS
NUCLEONS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
THERAPY