Resting lymphocyte transduction with measles virus glycoprotein pseudotyped lentiviral vectors relies on CD46 and SLAM

Journal Article · Tue May 10 00:00:00 EDT 2011 · Virology

DOI:https://doi.org/10.1016/j.virol.2011.02.010· OSTI ID:21486935

Qi, Zhou; Schneider, Irene C; Gallet, Manuela; Kneissl, Sabrina

The measles virus (MV) glycoproteins hemagglutinin (H) and fusion (F) were recently shown to mediate transduction of resting lymphocytes by lentiviral vectors. MV vaccine strains use CD46 or signaling lymphocyte activation molecule (SLAM) as receptor for cell entry. A panel of H protein mutants derived from vaccine strain or wild-type MVs that lost or gained CD46 or SLAM receptor usage were investigated for their ability to mediate gene transfer into unstimulated T lymphocytes. The results demonstrate that CD46 is sufficient for efficient vector particle association with unstimulated lymphocytes. For stable gene transfer into these cells, however, both MV receptors were found to be essential.

• OSTI ID: 21486935
• Journal Information: Virology, Vol. 413, Issue 2; Other Information: DOI: 10.1016/j.virol.2011.02.010; PII: S0042-6822(11)00071-7; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
GENES
GLYCOPROTEINS
HEMAGGLUTININS
LYMPHOCYTES
MEASLES VIRUS
MUTANTS
RECEPTORS
STRAINS
VACCINES
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
SACCHARIDES
SOMATIC CELLS
VIRUSES