Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein
- Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032 (United States)
- Arboviral Diseases Branch, Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, 3150 Rampart Rd., Fort Collins, CO 80521 (United States)
- Department of Microbiology, Immunology, and Pathology, Colorado State University, For Collins, CO 80523 (United States)
Hybridoma cell lines (2E8, 8G8 and 5G12) producing fully human monoclonal antibodies (hMAbs) specific for the pre-membrane (prM) protein of West Nile virus (WNV) were prepared using a human fusion partner cell line, MFP-2, and human peripheral blood lymphocytes from a blood donor diagnosed with WNV fever in 2004. Using site-directed mutagenesis of a WNV-like particle (VLP) we identified 4 amino acid residues in the prM protein unique to WNV and important in the binding of these hMAbs to the VLP. Residues V19 and L33 are important epitopes for the binding of all three hMAbs. Mutations at residue, T20 and T24 affected the binding of hMAbs, 8G8 and 5G12 only. These hMAbs did not significantly protect AG129 interferon-deficient mice or Swiss Webster outbred mice from WNV infection.
- OSTI ID:
- 21484508
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 1 Vol. 410; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
FEVER
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MICROORGANISMS
MONOCLONAL ANTIBODIES
MUTAGENESIS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RESIDUES
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
FEVER
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MICROORGANISMS
MONOCLONAL ANTIBODIES
MUTAGENESIS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RESIDUES
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES
VIRUSES