DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus
- Department of Biological Science, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore)
- Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411 (Singapore)
- Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore 117542 (Singapore)
The White Spot Syndrome Virus (WSSV) has a large circular double-stranded DNA genome of around 300 kb and it replicates in the nucleus of the host cells. The machinery of how the viral DNA is packaged has been remained unclear. VP15, a highly basic protein, is one of the major capsid proteins found in the virus. Previously, it was shown to be a DNA binding protein and was hypothesized to participate in the viral DNA packaging process. Using Atomic Force Microscopy imaging, we show that the viral DNA is associated with a (or more) capsid proteins. The organized viral DNA qualitatively resembles the conformations of VP15 induced DNA condensates in vitro. Furthermore, single-DNA manipulation experiments revealed that VP15 is able to condense single DNA against forces of a few pico Newtons. Our results suggest that VP15 may aid in the viral DNA packaging process by directly condensing DNA.
- OSTI ID:
- 21484506
- Journal Information:
- Virology, Vol. 408, Issue 2; Other Information: DOI: 10.1016/j.virol.2010.09.008; PII: S0042-6822(10)00583-0; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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