HIV-1 Gag p17 presented as virus-like particles on the E2 scaffold from Geobacillus stearothermophilus induces sustained humoral and cellular immune responses in the absence of IFN{gamma} production by CD4+ T cells
- Institute of Protein Biochemistry, C.N.R., via P. Castellino 111, 80131 Naples (Italy)
- Seattle Biomedical Research Institute, 307 Westlake Av, Seattle, WA 98109-5240 (United States)
We have constructed stable virus-like particles displaying the HIV-1 Gag(p17) protein as an N-terminal fusion with an engineered protein domain from the Geobacillus stearothermophilus pyruvate dehydrogenase subunit E2. Mice immunized with the Gag(p17)-E2 60-mer scaffold particles mounted a strong and sustained antibody response. Antibodies directed to Gag(p17) were boosted significantly with additional immunizations, while anti-E2 responses reached a plateau. The isotype of the induced antibodies was biased towards IgG1, and the E2-primed CD4+ T cells did not secrete IFN{gamma}. Using transgenic mouse model systems, we demonstrated that CD8+ T cells primed with E2 particles were able to exert lytic activity and produce IFN{gamma}. These results show that the E2 scaffold represents a powerful vaccine delivery system for whole antigenic proteins or polyepitope engineered proteins, evoking antibody production and antigen specific CTL activity even in the absence of IFN{gamma}-producing CD4+ T cells.
- OSTI ID:
- 21460286
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 2 Vol. 407; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood
Antigen presentation by small intestinal epithelial cells uniquely enhances IFN-γ secretion from CD4{sup +} intestinal intraepithelial lymphocytes
Journal Article
·
Sun Oct 10 04:00:00 UTC 2010
· Virology
·
OSTI ID:21417250
Antigen presentation by small intestinal epithelial cells uniquely enhances IFN-γ secretion from CD4{sup +} intestinal intraepithelial lymphocytes
Journal Article
·
Fri Jun 14 04:00:00 UTC 2013
· Biochemical and Biophysical Research Communications
·
OSTI ID:22239625