Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina

Journal Article · Sun Oct 10 04:00:00 EDT 2010 · Virology

DOI:https://doi.org/10.1016/j.virol.2010.07.002· OSTI ID:21460277

Leach, Natalie R ^[1]

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1. Program in Molecular and Cellular Biology, University of Iowa, Iowa City, IA 52242 (United States)

The nuclear lamina is thought to be a steric barrier to the herpesvirus capsid. Disruption of the lamina accompanied by phosphorylation of lamina proteins is a conserved feature of herpesvirus infection. In HSV-1-infected cells, protein kinase C (PKC) alpha and delta isoforms are recruited to the nuclear membrane and PKC delta has been implicated in phosphorylation of emerin and lamin B. We tested two critical hypotheses about the mechanism and significance of lamina disruption. First, we show that chemical inhibition of all PKC isoforms reduced viral growth five-fold and inhibited capsid egress from the nucleus. However, specific inhibition of either conventional PKCs or PKC delta does not inhibit viral growth. Second, we show hyperphosphorylation of emerin by viral and cellular kinases is required for its disassociation from the lamina. These data support hypothesis that phosphorylation of lamina components mediates lamina disruption during HSV nuclear egress.

• OSTI ID: 21460277
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 406; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CHEMICAL REACTIONS
DISEASES
ENZYMES
GROWTH
HERPES SIMPLEX
HOST
INFECTIOUS DISEASES
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
SKIN DISEASES
TRANSFERASES
VIRAL DISEASES
VIRUSES