Mono-hydroxy methoxychlor alters levels of key sex steroids and steroidogenic enzymes in cultured mouse antral follicles

Journal Article · Wed Dec 01 00:00:00 EST 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2010.09.001· OSTI ID:21460244

Craig, Zelieann R., E-mail: [e-mail] ^[1]; Leslie, Traci C., E-mail: [e-mail] ^[1]; Hatfield, Kimberly P., E-mail: [e-mail] ^[2]; Gupta, Rupesh K., E-mail: [e-mail] ^[1]; Flaws, Jodi A., E-mail: [e-mail] ^[1]

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1. Department of Comparative Biosciences, University of Illinois, Urbana, IL (United States)
2. Program in Toxicology and Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, MD 21201 (United States)

Methoxychlor (MXC) is an organochlorine pesticide that reduces fertility in female rodents by decreasing antral follicle numbers and increasing follicular death. MXC is metabolized in the body to mono-hydroxy MXC (mono-OH). Little is known about the effects of mono-OH on the ovary. Thus, this work tested the hypothesis that mono-OH exposure decreases production of 17{beta}-estradiol (E{sub 2}) by cultured mouse antral follicles. Antral follicles were isolated from CD-1 mice (age 35-39 days) and exposed to dimethylsulfoxide (DMSO), or mono-OH (0.1-10 {mu}g/mL) for 96 h. Media and follicles were collected for analysis of sex steroid levels and mRNA expression, respectively. Mono-OH treatment (10 {mu}g/mL) decreased E{sub 2} (DMSO: 3009.72 {+-} 744.99 ng/mL; mono-OH 0.1 {mu}g/mL: 1679.66 {+-} 461.99 ng/mL; 1 {mu}g/mL: 1752.72 {+-} 532.41 ng/mL; 10 {mu}g/mL: 45.89 {+-} 33.83 ng/mL), testosterone (DMSO: 15.43 {+-} 2.86 ng/mL; mono-OH 0.1 {mu}g/mL: 17.17 {+-} 4.71 ng/mL; 1 {mu}g/mL: 13.64 {+-} 3.53 ng/mL; 10 {mu}g/mL: 1.29 {+-} 0.23 ng/mL), androstenedione (DMSO: 1.92 {+-} 0.34 ng/mL; mono-OH 0.1 {mu}g/mL: 1.49 {+-} 0.43 ng/mL; 1 {mu}g/mL: 0.64 {+-} 0.31 ng/mL; 10 {mu}g/mL: 0.12 {+-} 0.06 ng/mL) and progesterone (DMSO: 24.11 {+-} 4.21 ng/mL; mono-OH 0.1 {mu}g/mL: 26.77 {+-} 4.41 ng/mL; 1 {mu}g/mL: 20.90 {+-} 3.75 ng/mL; 10 {mu}g/mL: 9.44 {+-} 2.97 ng/mL) levels. Mono-OH did not alter expression of Star, Hsd3b1, Hsd17b1 and Cyp1b1, but it did reduce levels of Cyp11a1, Cyp17a1 and Cyp19a1 mRNA. Collectively, these data suggest that mono-OH significantly decreases levels of key sex steroid hormones and the expression of enzymes required for steroidogenesis.

• OSTI ID: 21460244
• Journal Information: Toxicology and Applied Pharmacology, Vol. 249, Issue 2; Other Information: DOI: 10.1016/j.taap.2010.09.001; PII: S0041-008X(10)00331-5; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DMSO
ENZYMES
ESTRADIOL
FEMALES
FERTILITY
MESSENGER-RNA
METABOLITES
MICE
ORGANIC CHLORINE COMPOUNDS
OVARIES
PESTICIDES
PROGESTERONE
TESTOSTERONE
ANDROGENS
ANDROSTANES
ANIMALS
BODY
ESTRANES
ESTROGENS
FEMALE GENITALS
GONADS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RNA
RODENTS
STEROID HORMONES
STEROIDS
SULFOXIDES
VERTEBRATES