Trastuzumab-binding peptide display by Tobacco mosaic virus
- A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University and N.I. Vavilov Institute of General Genetics, Russian Academy of Science, Moscow 119992 (Russian Federation)
- Blokhin Cancer State Research Center, Russian Academy of Medical Sciences, Moscow (Russian Federation)
- Nomad Bioscience GmbH, Biozentrum Halle, Weinbergweg 22, D-06120 Halle (Saale) (Germany)
Human epidermal growth factor receptor-2 (HER2/neu) is a target for the humanized monoclonal antibody trastuzumab. Recently, trastuzumab-binding peptides (TBP) of HER2/neu that inhibit proliferation of breast cancer cells were identified. We have now studied conditions of efficient assembly in vivo of Tobacco mosaic virus (TMV)-based particles displaying TBP on its surface. The system is based on an Agrobacterium-mediated co-delivery of binary vectors encoding TMV RNA and coat protein (CP) with TBP in its C-terminal extension into plant leaves. We show how the fusion of amino acid substituted TBP (sTBP) to CP via a flexible peptide linker can improve the manufacturability of recombinant TMV (rTMV). We also reveal that rTMV particles with exposed sTBP retained trastuzumab-binding capacity but lost an anti-HER2/neu immunogenic scaffold function. Mouse antibodies against rTMV did not recognize HER2/neu on surface of human SK-BR-3 cells.
- OSTI ID:
- 21417252
- Journal Information:
- Virology, Vol. 407, Issue 1; Other Information: DOI: 10.1016/j.virol.2010.08.005; PII: S0042-6822(10)00522-2; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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