Microangiography in Living Mice Using Synchrotron Radiation

Journal Article · Fri Jul 23 00:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3478201· OSTI ID:21415287

Falei, Yuan; Yongting, Wang; Bohua, Xie; Yaohui, Tang ^[1]; Yongjing, Guan ^[1]; Haiyan, Lu; Guoyuan, Yang ^[1]; Honglan, Xie; Guohao, Du; Tiqiao, Xiao ^[2]

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1. Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030 (China)
2. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai (China)

Traditionally, there are no methods available to detect the fine morphologic changes of cerebrovasculature in small living animals such as rats and mice. Newly developed synchrotron radiation microangiography can achieve a fine resolution of several micrometers and had provided us with a powerful tool to study the cerebral vasculature in small animals. The purpose of this study is to identify the morphology of cerebrovasculature especially the structure of Lenticulostriate arteries (LSAs) in living mice using the synchrotron radiation source at Shanghai Synchrotron Radiation Facility (SSRF) in Shanghai, China. Adult CD-1 mice weighing 35-40 grams were anesthetized. Nonionic iodine (Omnipaque, 350 mg I /mL) was used as a contrast agent. The study was performed at the BL13W1 beam line at SSRF. The beam line was derived from a storage ring of electrons with an accelerated energy of 3.5 GeV and an average beam current of 200 mA. X-ray energy of 33.3 keV was used to produce the highest contrast image. Images were acquired every 172 ms by a x-ray camera (Photonic-Science VHR 1.38) with a resolution of 13 {mu}m/pixel. The optimal dose of contrast agent is 100 {mu}l per injection and the injecting rate is 33 {mu}l/sec. The best position for imaging is to have the mouse lay on its right or left side, with ventral side facing the X-ray source. We observed the lenticulostriate artery for the first time in living mice. Our result show that there are 4 to 5 lenticulostriate branches originating from the root of middle cerebral artery in each hemisphere. LSAs have an average diameter of 43{+-}6.8 {mu}m. There were no differences between LSAs from the left and right hemisphere (p<0.05). These results suggest that synchrotron radiation may provide a unique tool for experimental stroke research.

• OSTI ID: 21415287
• Journal Information: AIP Conference Proceedings, Vol. 1266, Issue 1; Conference: 6. international conference on medical applications of synchrotron radiation, Melbourne (Australia), 15-18 Feb 2010; Other Information: DOI: 10.1063/1.3478201; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BEAM CURRENTS
BIOLOGICAL RADIATION EFFECTS
CEREBRAL ARTERIES
CHARGE-COUPLED DEVICES
CONTRAST MEDIA
GEV RANGE 01-10
IMAGE PROCESSING
IODINE
KEV RANGE 10-100
MICE
MORPHOLOGY
RADIATION DOSES
RATS
RESOLUTION
SYNCHROTRON RADIATION
SYNCHROTRON RADIATION SOURCES
TOMOGRAPHY
X RADIATION
X-RAY SOURCES
ANIMALS
ARTERIES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
BREMSSTRAHLUNG
CARDIOVASCULAR SYSTEM
CURRENTS
DIAGNOSTIC TECHNIQUES
DOSES
ELECTROMAGNETIC RADIATION
ELEMENTS
ENERGY RANGE
GEV RANGE
HALOGENS
IONIZING RADIATIONS
KEV RANGE
MAMMALS
NONMETALS
ORGANS
PROCESSING
RADIATION EFFECTS
RADIATION SOURCES
RADIATIONS
RODENTS
SEMICONDUCTOR DEVICES
VERTEBRATES