Irradiation With Carbon Ion Beams Induces Apoptosis, Autophagy, and Cellular Senescence in a Human Glioma-Derived Cell Line
Journal Article
·
· International Journal of Radiation Oncology, Biology and Physics
- Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma (Japan)
- 21st Century Center of Excellence Program for Biomedical Research Using Accelerator Technology, Maebashi, Gunma (Japan)
- Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma (Japan)
- Microbeam Radiation Biology Group, Radiation-Applied Biology Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma (Japan)
Purpose: We examined biological responses of human glioma cells to irradiation with carbon ion beams (C-ions). Methods and Materials: A human glioma-derived cell line, NP-2, was irradiated with C-ions. Apoptotic cell nuclei were stained with Hoechst 33342. Induction of autophagy was examined either by staining cells with monodansylcadaverine (MDC) or by Western blotting to detect conversion of microtuble-associated protein light chain 3 (MAP-LC3) (LC3-I) to the membrane-bound form (LC3-II). Cellular senescence markers including induction of senescence-associated beta-galactosidase (SA-beta-gal) were examined. The mean telomere length of irradiated cells was determined by Southern blot hybridization. Expression of tumor suppressor p53 and cyclin/cyclin-dependent kinase inhibitor p21{sup WAF1/CIP1} in the irradiated cells was analyzed by Western blotting. Results: When NP-2 cells were irradiated with C-ions at 6 Gy, the major population of the cells died of apoptosis and autophagy. The residual fraction of attached cells (<1% of initially irradiated cells) could not form a colony: however, they showed a morphological phenotype consistent with cellular senescence, that is, enlarged and flattened appearance. The senescent nature of these attached cells was further indicated by staining for SA-beta-gal. The mean telomere length was not changed after irradiation with C-ions. Phosphorylation of p53 at serine 15 as well as the expression of p21{sup WAF1/CIP1} was induced in NP-2 cells after irradiation. Furthermore, we found that irradiation with C-ions induced cellular senescence in a human glioma cell line lacking functional p53. Conclusions: Irradiation with C-ions induced apoptosis, autophagy, and cellular senescence in human glioma cells.
- OSTI ID:
- 21367610
- Journal Information:
- International Journal of Radiation Oncology, Biology and Physics, Journal Name: International Journal of Radiation Oncology, Biology and Physics Journal Issue: 1 Vol. 76; ISSN IOBPD3; ISSN 0360-3016
- Country of Publication:
- United States
- Language:
- English
Similar Records
Dihydroptychantol A, a macrocyclic bisbibenzyl derivative, induces autophagy and following apoptosis associated with p53 pathway in human osteosarcoma U2OS cells
AMPK induces vascular smooth muscle cell senescence via LKB1 dependent pathway
Central role of mitochondria and p53 in PUVA-induced apoptosis in human keratinocytes cell line NCTC-2544
Journal Article
·
Mon Feb 28 23:00:00 EST 2011
· Toxicology and Applied Pharmacology
·
OSTI ID:21535247
AMPK induces vascular smooth muscle cell senescence via LKB1 dependent pathway
Journal Article
·
Fri Sep 16 00:00:00 EDT 2011
· Biochemical and Biophysical Research Communications
·
OSTI ID:22207500
Central role of mitochondria and p53 in PUVA-induced apoptosis in human keratinocytes cell line NCTC-2544
Journal Article
·
Thu Feb 14 23:00:00 EST 2008
· Toxicology and Applied Pharmacology
·
OSTI ID:21077924