The Elav-like protein HuR exerts translational control of viral internal ribosome entry sites
- Laboratorio de Virologia Molecular, Instituto Milenio de Inmunologia e Inmunoterapia, Jeune Equipe Associee a l'IRD, Centro de Investigaciones Medicas, Facultad de Medicina, Pontificia Universidad Catolica de Chile, Marcoleta 391, Santiago (Chile)
- Department of Biochemistry, Faculty of Medicine, McGill University, Montreal, Quebec (Canada)
- Centro de Regulacion Celular y Patologia, J. V. Luco e Instituto Milenio de Biologia Fundamental y Aplicada, MIFAB, Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago (Chile)
The human embryonic-lethal abnormal vision (ELAV)-like protein, HuR, has been recently found to be involved in the regulation of protein synthesis. In this study we show that HuR participates in the translational control of the HIV-1 and HCV IRES elements. HuR functions as a repressor of HIV-1 IRES activity and acts as an activator of the HCV IRES. The effect of HuR was evaluated in three independent experimental systems, rabbit reticulocyte lysate, HeLa cells, and Xenopus laevis oocytes, using both overexpression and knockdown approaches. Furthermore, results suggest that HuR mediated regulation of HIV-1 and HCV IRESes does not require direct binding of the protein to the RNA nor does it need the nuclear translocation of the IRES-containing RNAs. Finally, we show that HuR has a negative impact on post-integration steps of the HIV-1 replication cycle. Thus, our observations yield novel insights into the role of HuR in the post-transcriptional regulation of HCV and HIV-1 gene expression.
- OSTI ID:
- 21357542
- Journal Information:
- Virology, Vol. 392, Issue 2; Other Information: DOI: 10.1016/j.virol.2009.06.050; PII: S0042-6822(09)00412-7; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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