The role of the PI3K-Akt signal transduction pathway in Autographa californica multiple nucleopolyhedrovirus infection of Spodoptera frugiperda cells

Journal Article · Sat Aug 15 00:00:00 EDT 2009 · Virology

DOI:https://doi.org/10.1016/j.virol.2009.06.007· OSTI ID:21357534

Wei, Xiao; Yi, Yang; Qingbei, Weng; Tiehao, Lin; Meijin, Yuan ^[1]; Yi, Pang ^[1]

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1. State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275 (China)

Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt pathway in Baculovirus replication has not been defined. In the present study, we demonstrate that infection of Sf9 cells with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) elevated cellular Akt phosphorylation at 1 h post-infection. The maximum Akt phosphorylation occurred at 6 h post-infection and remained unchanged until 18 h post-infection. The PI3K-specific inhibitor, LY294002, suppressed Akt phosphorylation in a dose-dependent manner, suggesting that AcMNPV-induced Akt phosphorylation is PI3K-dependent. The inhibition of PI3K-Akt activation by LY294002 significantly reduced the viral yield, including a reduction in budded viruses and occlusion bodies. The virus production was reduced only when the inhibitor was added within 24 h of infection, implying that activation of PI3K occurred early in infection. Correspondingly, both viral DNA replication and late (VP39) and very late (POLH) viral protein expression were impaired by LY294002 treatment; LY294002 had no effect on immediate-early (IE1) and early-late (GP64) protein expression. These results demonstrate that the PI3K-Akt pathway is required for efficient Baculovirus replication.

• OSTI ID: 21357534
• Journal Information: Virology, Vol. 391, Issue 1; Other Information: DOI: 10.1016/j.virol.2009.06.007; PII: S0042-6822(09)00351-1; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DNA REPLICATION
GLUCOSE
METABOLISM
MOTHS
PHOSPHORYLATION
PROTEINS
SIGNALS
SYNTHESIS
VIRUSES
ALDEHYDES
ANIMALS
ARTHROPODS
CARBOHYDRATES
CHEMICAL REACTIONS
HEXOSES
INSECTS
INVERTEBRATES
LEPIDOPTERA
MICROORGANISMS
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PARASITES
SACCHARIDES