Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells

Journal Article · Sat May 01 00:00:00 EDT 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2010.01.007· OSTI ID:21344933

Chen, C -Y ^[1]

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1. Department of Microbiology, Soochow University, Shihlin, Taipei, Taiwan (China)

Butyl benzyl phthalate (BBP), an endocrine disruptor present in the environment, exerts its genomic effects via intracellular steroid receptors and elicits non-genomic effects by interfering with membrane ion-channel receptors. We previously found that BBP blocks the calcium signaling coupled with P2X receptors in PC12 cells (Liu and Chen, 2006). Osteoblast P2X receptors were recently reported to play a role in cell proliferation and bone remodeling. In this present study, the effects of BBP on ATP-induced responses were investigated in human osteosarcoma HOS cells. These receptors mRNA had been detected, named P2X4, P2X7, P2Y2, P2Y4, P2Y5, P2Y9, and P2Y11, in human osteosarcoma HOS cells by RT-PCR. The enhancement of cell proliferation and the decrease of cytoviability had both been shown to be coupled to stimulation via different concentrations of ATP. BBP suppressed the ATP-induced calcium influx (mainly coupled with P2X) and cell proliferation but not the ATP-induced intracellular calcium release (mainly coupled with P2Y) and cytotoxicity in human osteosarcoma HOS cells. Suramin, a common P2 receptor's antagonist, blocked the ATP-induced calcium signaling, cell proliferation, and cytotoxicity. We suggest that P2X is mainly responsible for cell proliferation, and P2Y might be partially responsible for the observed cytotoxicity. BBP suppressed the calcium signaling coupled with P2X, suppressing cell proliferation. Since the importance of P2X receptors during bone metastasis has recently become apparent, the possible toxic risk of environmental BBP during bone remodeling is a public problem of concern.

• OSTI ID: 21344933
• Journal Information: Toxicology and Applied Pharmacology, Vol. 244, Issue 3; Other Information: DOI: 10.1016/j.taap.2010.01.007; PII: S0041-008X(10)00018-9; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATP
CALCIUM
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
HAZARDS
MEN
MESSENGER-RNA
METASTASES
OSTEOSARCOMAS
PHTHALATES
RECEPTORS
SKELETON
STEROIDS
STIMULATION
TOXICITY
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACID SALTS
DISEASES
ELEMENTS
MALES
MAMMALS
MAN
MEMBRANE PROTEINS
METALS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RNA
SARCOMAS
SKELETAL DISEASES
SOMATIC CELLS
VERTEBRATES