Characterization of function and regulation of miR-24-1 and miR-31

Journal Article · Fri Mar 13 04:00:00 EDT 2009 · Biochemical and Biophysical Research Communications

OSTI ID:21255933

Fenyong, Sun ^[1]; Jiayi, Wang ^[2]; Qiuhui, Pan ^[3]; Yongchun, Yu ^[2]; Yue, Zhang; Yang, Wan; Ju, Wang ^[1]; Xiaoyan, Li ^[2]

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1. Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou City, 510632 Guangdong Province (China)
2. Department of Laboratory Centre, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072 (China)
3. Medical Research Centre, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou City, 510120 Guangdong Province (China)

To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b{approx}27b{approx}24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.

• OSTI ID: 21255933
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 380; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CYCLE
CELL PROLIFERATION
GENE REGULATION
GENES
MICE
PROMOTERS
STEM CELLS
TRANSCRIPTION