NF-{kappa}B p50 promotes tumor cell invasion through negative regulation of invasion suppressor gene CRMP-1 in human lung adenocarcinoma cells

Journal Article · Fri Nov 14 04:00:00 EST 2008 · Biochemical and Biophysical Research Communications

OSTI ID:21217096

Ming, Gao ^[1]; Yeh, P Y ^[1]; Lu, Y -S ^[1]; Chang, W C ^[1]; Kuo, M -L ^[2]; Cheng, A -L ^[1]

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1. Cancer Research Center, College of Medicine, National Taiwan University, Taipei, Taiwan (China)
2. Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China)

Lung adenocarcinoma Cl1-5 cells were selected from parental Cl1-0 cells based on their high metastatic potential. In a previous study, CRMP-1, an invasion suppressor gene, was shown to be suppressed in Cl1-5 cells. However, the regulation of CRMP-1 expression has not been explored. In this study, we showed nuclear factor-{kappa}B controls CRMP-1 expression. The electromobility shift assay showed that while Cl1-0 cells exhibited low NF-{kappa}B activity in response to TNF-{alpha}, an abundance of basal and TNF-{alpha}-induced NF-{kappa}B-DNA complex was detected in Cl1-5 cells. Supershift-coupled EMSA and Western blotting of nuclear proteins, however, revealed p50 protein, but not classic p65/p50 heterodimer in the complex. ChIP and EMSA demonstrated that p50 binds to a {kappa}B site residing between -1753 and -1743 of the CRMP-1 promoter region. Transfection of antisense p50 gene into Cl1-5 cells increased the CRMP-1 protein level and decreased the invasive activity of Cl1-5 cells.

• OSTI ID: 21217096
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 376; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CARCINOMAS
CHROMATIN
DNA
GENES
HUMAN POPULATIONS
LUNGS
METASTASES
PROTEINS
REGULATIONS
TUMOR CELLS
TUMOR PROMOTERS