Mucosal prior to systemic application of recombinant adenovirus boosting is more immunogenic than systemic application twice but confers similar protection against SIV-challenge in DNA vaccine-primed macaques

Journal Article · Tue Jan 20 04:00:00 EST 2009 · Virology

DOI:https://doi.org/10.1016/j.virol.2008.10.012· OSTI ID:21182798

Schulte, Reiner; Suh, You-Suk; Sauermann, Ulrike; Ochieng, Washingtone ^[1]; Sopper, Sieghart ^[2]; Kim, Kwang S; Ahn, So-Shin; Park, Ki S ^[3]; Stolte-Leeb, Nicole ^[1]; Hunsmann, Gerhard ^[2]; Stahl-Hennig, Christiane ^[1]

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1. Working group 'Infection Models', German Primate Center, Kellnerweg 4, 37077, Goettingen (Germany)
2. Department of Virology and Immunology, German Primate Center, Kellnerweg 4, 37077, Goettingen (Germany)
3. Cellular Immunology Laboratory, Division of Molecular and Life Sciences, Pohang University of Science and Technology (Korea, Republic of)

We investigated the immunogenicity and efficacy of a bimodal prime/boost vaccine regimen given by various routes in the Simian immunodeficiency virus (SIV) rhesus monkey model for AIDS. Twelve animals were immunized with SIV DNA-vectors followed by the application of a recombinant adenovirus (rAd5) expressing the same genes either intramuscularly (i.m.) or by oropharyngeal spray. The second rAd5-application was given i.m. All vaccinees plus six controls were challenged orally with SIVmac239 12 weeks post-final immunization. Both immunization strategies induced strong SIV Gag-specific IFN-{gamma} and T-cell proliferation responses and mediated a conservation of CD4{sup +} memory T-cells and a reduction of viral load during peak viremia following infection. Interestingly, the mucosal group was superior to the systemic group regarding breadth and strength of SIV-specific T-cell responses and exhibited lower vector specific immune responses. Therefore, our data warrant the inclusion of mucosal vector application in a vaccination regimen which makes it less invasive and easier to apply.

• OSTI ID: 21182798
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 383; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADENOVIRUS
AIDS
CELL PROLIFERATION
DNA
GENES
MACACUS
VACCINES