The structural biology center at the APS: an integrated user facility for macromolecular crystallography

Journal Article · Tue Jul 01 04:00:00 EDT 1997 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.54578· OSTI ID:21179542

Rosenbaum, G; Westbrook, E M ^[1]

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1. Structural Biology Center, Argonne National Laboratory, Advanced Photon Source, 9700 S. Cass Ave., Argonne, Illinois 60439 (United States)

The Structural Biology Center (SBC) has developed and operates a sector (undulator and bending magnet) of the APS as a user facility for macromolecular crystallography. Crystallographically determined structures of proteins, nucleic acids and their complexes with proteins, viruses, and complexes between macromolecules and small ligands have become of central importance in molecular and cellular biology. Major design goals were to make the extremely high brilliance of the APS available for brilliance limited studies, and to achieve a high throughput of less demanding studies, as well as optimization for MAS-phasing. Crystal samples will include extremely small crystals, crystals with large unit cells (viruses, ribosomes, etc.) and ensembles of closely similar crystal structures for drug design, protein engineering, etc. Data are recorded on a 3000x3000 pixel CCD-area detector (optionally on image plates). The x-ray optics of both beamlines has been designed to produce a highly demagnified image of the source in order to match the focal size with the sizes of the sample and the resolution element of the detector. Vertical focusing is achieved by a flat, cylindrically bent mirror. Horizontal focusing is achieved by sagitally bending the second crystal of the double crystal monochromator. Monochromatic fluxes of 1.3*10{sup 13} ph/s into focal sizes of 0.08 mm (horizontal)x0.04 mm (vertical) FWHM (flux density 3.5*10{sup 15} ph/s/mm{sup 2}) have been recorded.

• OSTI ID: 21179542
• Journal Information: AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 417; ISSN APCPCS; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

43 PARTICLE ACCELERATORS
60 APPLIED LIFE SCIENCES
ADVANCED PHOTON SOURCE
BEAM OPTICS
CHARGE-COUPLED DEVICES
CRYSTAL STRUCTURE
CRYSTALS
DRUGS
FLUX DENSITY
IMAGES
MOLECULAR BIOLOGY
NUCLEIC ACIDS
PHOTON BEAMS
PROTEIN ENGINEERING
PROTEINS
RIBOSOMES
VIRUSES
WIGGLER MAGNETS
X-RAY DIFFRACTION