TGF-{beta}'s delay skeletal muscle progenitor cell differentiation in an isoform-independent manner

Journal Article · Sun Feb 01 04:00:00 EST 2009 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2008.10.037· OSTI ID:21176165

Schabort, Elske J ^[1]; Merwe, Mathilde van der ^[2]; Loos, Benjamin; Moore, Frances P ^[1]

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1. Department of Physiological Sciences, University of Stellenbosch, Private Bag X1, Stellenbosch 7602 (South Africa)
2. Department of Genetics, University of Stellenbosch, Private Bag X1, Stellenbosch 7602 (South Africa)

Satellite cells are a quiescent heterogenous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-{beta} (TGF-{beta}) superfamily members are elevated post-injury and their importance in the regulation of myogenesis and wound healing has been demonstrated both in vitro and in vivo. Most studies suggest a negative role for TGF-{beta} on satellite cell differentiation. However, none have compared the effect of these three isoforms on myogenesis in vitro. This is despite known isoform-specific effects of TGF-{beta}1, -{beta}2 and -{beta}3 on wound repair in other tissues. In the current study we compared the effect of TGF-{beta}1, -{beta}2 and -{beta}3 on proliferation and differentiation of the C2C12 myoblast cell-line. We found that, irrespective of the isoform, TGF-{beta} increased proliferation of C2C12 cells by changing the cellular localisation of PCNA to promote cell division and prevent cell cycle exit. Concomitantly, TGF-{beta}1, -{beta}2 and -{beta}3 delayed myogenic commitment by increasing MyoD degradation and decreasing myogenin expression. Terminal differentiation, as measured by a decrease in myosin heavy chain (MHC) expression, was also delayed. These results demonstrate that TGF-{beta} promotes proliferation and delays differentiation of C2C12 myoblasts in an isoform-independent manner.

• OSTI ID: 21176165
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 3 Vol. 315; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CYCLE
CELL DIFFERENTIATION
CELL DIVISION
GROWTH FACTORS
IN VITRO
IN VIVO
MYOBLASTS
MYOSIN
WOUNDS