Evidence for {gamma}-actin as a Z disc component in skeletal myofibers

Journal Article · Thu Jan 15 04:00:00 EST 2009 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2008.10.021· OSTI ID:21176158

Papponen, Hinni; Kaisto, Tuula; Kaakinen, Mika ^[1]; Metsikkoe, Kalervo ^[1]

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1. Institute of Biomedicine, Department of Anatomy and Cell Biology, P.O. Box 5000 (Aapistie 7), FIN-90014 University of Oulu (Finland)

We investigated the targeting of the {gamma}-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged {gamma}-actin in rat flexor digitorum brevis myofibers. We found that the {gamma}-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific {alpha}-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the {gamma}-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of {gamma}-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells.

• OSTI ID: 21176158
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 315; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
GLYCOPROTEINS
MICROTUBULES
MUSCLES
RATS