The Cu-Zn superoxide dismutase (SOD1) inhibits ERK phosphorylation by muscarinic receptor modulation in rat pituitary GH3 cells

Journal Article · Fri Nov 07 04:00:00 EST 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.08.110· OSTI ID:21146679

Secondo, Agnese ^[1]; De Mizio, Mariarosaria; Zirpoli, Laura; Santillo, Mariarosaria ^[2]; Mondola, Paolo ^[2]

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1. Department of Neuroscience, Section of Pharmacology, University of Naples 'Federico II', Via S. Pansini, 5, 80131 Naples (Italy)
2. Department of Neuroscience, Section of Physiology, University of Naples 'Federico II', Via S. Pansini, 5, 80131 Naples (Italy)

The Cu-Zn superoxide dismutase (SOD1) belongs to a family of isoenzymes that are able to dismutate the oxygen superoxide in hydrogen peroxide and molecular oxygen. This enzyme is secreted by many cellular lines and it is also released trough a calcium-dependent depolarization mechanism involving SNARE protein SNAP 25. Using rat pituitary GH3 cells that express muscarinic receptors we found that SOD1 inhibits P-ERK1/2 pathway trough an interaction with muscarinic M1 receptor. This effect is strengthened by oxotremorine, a muscarinic M agonist and partially reverted by pyrenzepine, an antagonist of M1 receptor; moreover this effect is independent from increased intracellular calcium concentration induced by SOD1. Finally, P-ERK1/2 inhibition was accompanied by the reduction of GH3 cell proliferation. These data indicate that SOD1 beside the well studied antioxidant properties can be considered as a neuromodulator able to affect mitogen-activated protein kinase in rat pituitary cells trough a M1 muscarinic receptor.

• OSTI ID: 21146679
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 376; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIOXIDANTS
CALCIUM
CELL PROLIFERATION
DEPOLARIZATION
EDTA
ESTERS
HYDROGEN PEROXIDE
ISOENZYMES
PHOSPHORYLATION
RATS
RECEPTORS
SODIUM
SULFATES
SUPEROXIDE DISMUTASE