Low-resolution characterization of the 3D structure of the Euglena gracilis photoreceptor
- Istituto di Biofisica, Via Moruzzi 1, 56124 Pisa (Italy)
- Istituto Scienza Tecnologia Informazione, Via Moruzzi 1, 56124 Pisa (Italy)
- Istituto Fisiologia Clinica, Via Moruzzi 1, 56124 Pisa (Italy)
This paper deals with the first characterization of the structure of the photoreceptive organelle of the unicellular alga Euglena gracilis (Euglenophyta). This organelle has a three-dimensional organization consisting of up to 50 closely stacked membrane lamellae. Ionically induced unstacking of the photoreceptor lamellae revealed ordered arrays well suited to structural analysis by electron microscopy and image analysis, which ultimately yielded a low-resolution picture of the structure. Each lamella is formed by the photoreceptive membrane protein of the cell assembled within the membrane layer in a hexagonal lattice. The first order diffraction spots in the calculated Fourier transform reveals the presence of 6-fold symmetrized topography (better resolution about 90 A). The 2D and 3D structural data are very similar with those recently published on proteorodopsin, a membrane protein used by marine bacterio-plankton as light-driven proton pump. In our opinion these similarity indicate that a photoreceptive protein belonging to the same superfamily of proteorodopsin could form the Euglena photoreceptor.
- OSTI ID:
- 21143913
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 375, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2008.08.045; PII: S0006-291X(08)01588-X; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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