Neuronal differentiation modulates the dystrophin Dp71d binding to the nuclear matrix
- Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico)
- Departamento de Biologia Celular, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico)
- Departamento de Bioquimica, Centro de Investigacion y de Estudios Avanzados del IPN, Avenida Instituto Politecnico Nacional 2508, Apartado Postal 14-740, C.P. 07360, Mexico D.F. (Mexico)
- Facultad de Medicina, Universidad de Colima, Avenida Universidad 333. C.P. 28040 Colima, Col. (Mexico)
The function of dystrophin Dp71 in neuronal cells remains unknown. To approach this issue, we have selected the PC12 neuronal cell line. These cells express both a Dp71f cytoplasmic variant and a Dp71d nuclear isoform. In this study, we demonstrated by electron and confocal microscopy analyses of in situ nuclear matrices and Western blotting evaluation of cell extracts that Dp71d associates with the nuclear matrix. Interestingly, this binding is modulated during NGF-induced neuronal differentiation of PC12 cells with a twofold increment in the differentiated cells, compared to control cells. Also, distribution of Dp71d along the periphery of the nuclear matrix observed in the undifferentiated cells is replaced by intense fluorescent foci localized in Center of the nucleoskeletal structure. In summary, we revealed that Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation.
- OSTI ID:
- 21143907
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 375, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2008.07.135; PII: S0006-291X(08)01451-4; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isolation and characterization of neural stem cells from dystrophic mdx mouse
Characterization of a novel Dp71 dystrophin-associated protein complex (DAPC) present in the nucleus of HeLa cells: Members of the nuclear DAPC associate with the nuclear matrix