The potassium ion channel opener NS1619 inhibits proliferation and induces apoptosis in A2780 ovarian cancer cells

Journal Article · Fri Oct 17 04:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

OSTI ID:21143900

Xiaobing, Han ^[1]; Ling, Xi; Hui, Wang; Xiaoyuan, Huang; Xiangyi, Ma; Zhiqiang, Han; Peng, Wu; Xiaoli, Ma; Yunping, Lu ^[1]; Wang, Gang ^[2]; Jianfeng, Zhou ^[1]

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1. Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030 (China)
2. Department of Neurology and Neuroscience, Weill Medical College of Cornell University, NY (United States)

Diverse types of voltage-gated potassium (K{sup +}) channels have been shown to be involved in regulation of cell proliferation. The maxi-conductance Ca{sup 2+}-activated K{sup +} channels (BK channels) may play an important role in the progression of human cancer. To explore the role of BK channels in regulation of apoptosis in human ovarian cancer cells, the effects of the specific BK channel activator NS1619 on induction of apoptosis in A2780 cells were observed. Following treatment with NS1619, cell proliferation was measured by MTT assay. Apoptosis of A2780 cells pretreated with NS1619 was detected by agarose gel electrophoresis of cellular DNA and flow cytometry. Our data demonstrate that NS1619 inhibits the proliferation of A2780 cells in a dosage and time dependent manner IC{sub 50} = 31.1 {mu}M, for 48 h pretreatment and induces apoptosis. Western blot analyses showed that the anti-proliferation effect of NS1619 was associated with increased expression of p53, p21, and Bax. These results indicate that BK channels play an important role in regulating proliferation of human ovarian cancer cells and may induce apoptosis through induction of p21{sup Cip1} expression in a p53-dependent manner.

• OSTI ID: 21143900
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 375; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CALCIUM IONS
CELL PROLIFERATION
ELECTROPHORESIS
MONOCLINIC LATTICES
NEOPLASMS
POTASSIUM
POTASSIUM IONS