Nuclear deformation and expression change of cartilaginous genes during in vitro expansion of chondrocytes

Journal Article · Fri Oct 03 04:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.07.112· OSTI ID:21143887

Hoshiba, Takashi ^[1]; Yamada, Tomoe; Lu, Hongxu ^[1]; Kawazoe, Naoki; Tateishi, Tetsuya ^[1]; Chen, Guoping ^[1]

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1. Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan)

Cartilaginous gene expression decreased when chondrocytes were expanded on cell-culture plates. Understanding the dedifferentiation mechanism may provide valuable insight into cartilage tissue engineering. Here, we demonstrated the relationship between the nuclear shape and gene expression during in vitro expansion culture of chondrocytes. Specifically, the projected nuclear area increased and cartilaginous gene expressions decreased during in vitro expansion culture. When the nuclear deformation was recovered by cytochalasin D treatment, aggrecan expression was up-regulated and type I collagen (Col1a2) expression was down-regulated. These results suggest that nuclear deformation may be one of the mechanisms for chondrocyte dedifferentiation during in vitro expansion culture.

• OSTI ID: 21143887
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 374; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CARTILAGE
CELL CULTURES
COLLAGEN
GENES
IN VITRO
NUCLEAR DEFORMATION