Podocalyxin expression in malignant astrocytic tumors

Journal Article · Fri Sep 19 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.07.049· OSTI ID:21143872

Hayatsu, Norihito ^[1]; Kaneko, Mika Kato ^[2]; Mishima, Kazuhiko; Nishikawa, Ryo; Matsutani, Masao ^[3]; Price, Janet E ^[2]; Kato, Yukinari ^[2]

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1. Graduate School of Medicine, Kyoto University, Yoshida-konoe-cho, Sakyo-ku, Kyoto 606-8501 (Japan)
2. Department of Cancer Biology, Unit 173, University of Texas M.D. Anderson Cancer Center, 1515 Holcombe, Houston, TX 77030 (United States)
3. Saitama Medical University International Medical Center, 1397-1 Yamane Hidaka-shi, Saitama 350-1298 (Japan)

Podocalyxin is an anti-adhesive mucin-like transmembrane sialoglycoprotein that has been implicated in the development of aggressive forms of cancer. Podocalyxin is also known as keratan sulfate (KS) proteoglycan. Recently, we revealed that highly sulfated KS or another mucin-like transmembrane sialoglycoprotein podoplanin/aggrus is upregulated in malignant astrocytic tumors. The aim of this study is to examine the relationship between podocalyxin expression and malignant progression of astrocytic tumors. In this study, 51 astrocytic tumors were investigated for podocalyxin expression using immunohistochemistry, Western blot analysis, and quantitative real-time PCR. Immunohistochemistry detected podocalyxin on the surface of tumor cells in six of 14 anaplastic astrocytomas (42.9%) and in 17 of 31 glioblastomas (54.8%), especially around proliferating endothelial cells. In diffuse astrocytoma, podocalyxin expression was observed only in vascular endothelial cells. Podocalyxin might be associated with the malignant progression of astrocytic tumors, and be a useful prognostic marker for astrocytic tumors.

• OSTI ID: 21143872
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 374, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2008.07.049; PII: S0006-291X(08)01383-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ASTROCYTOMAS
CENTRAL NERVOUS SYSTEM
POLYMERASE CHAIN REACTION
SULFATES
TUMOR CELLS