A simple and efficient method for deriving neurospheres from bone marrow stromal cells

Journal Article · Fri Aug 08 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.05.039· OSTI ID:21143793

Qin, Yang; Jun, Mu; Qi, Li ^[1]; Ao, Li ^[2]; Zhilei, Zeng; Jun, Yang; Xiaodong, Zhang; Jin, Tang ^[1]

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1. Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education, 1 Yixueyuan Road, Chongqing 400016 (China)
2. Department of Pharmacology, Chongqing Medical University, Chongqing 400016 (China)

Bone marrow stromal cells (MSCs) can be differentiated into neuronal and glial-like cell types under appropriate experimental conditions. However, previously reported methods are complicated and involve the use of toxic reagents. Here, we present a simplified and nontoxic method for efficient conversion of rat MSCs into neurospheres that express the neuroectodermal marker nestin. These neurospheres can proliferate and differentiate into neuron, astrocyte, and oligodendrocyte phenotypes. We thus propose that MSCs are an emerging model cell for the treatment of a variety of neurological diseases.

• OSTI ID: 21143793
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 372, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.05.039; PII: S0006-291X(08)00923-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BONE MARROW
CELL DIFFERENTIATION
NERVE CELLS
NERVOUS SYSTEM DISEASES
PHENOTYPE
RATS
REAGENTS
TOXICITY