Thr308 determines Akt1 nuclear localization in insulin-stimulated keratinocytes

Journal Article · Fri Jul 18 04:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.04.185· OSTI ID:21143770

Goren, Itamar; Mueller, Elke; Pfeilschifter, Josef ^[1]; Frank, Stefan ^[1]

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1. pharmazentrum frankfurt/ZAFES, Institut fuer Allgemeine Pharmakologie und Toxikologie, Klinikum der Johann Wolfgang Goethe-Universitaet Frankfurt/M, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main (Germany)

Here, we determined the localization and activation of protein kinase B (Akt) in acute cutaneous wound tissue in mice. Akt1 represented the major Akt isoform that was expressed and activated in wound margin keratinocytes and also in the cultured human keratinocyte line HaCaT. Mutation of Akt1 protein, exchanging the activation-essential Ser473 and Thr308 residues for inactive Ala or phosphorylation-mimicking Asp and Glu residues, revealed that phosphorylation of Ser473 represented an essential prerequisite for auto-phosphorylation of Thr308 within the Akt1 protein in keratinocytes. Moreover, cell culture experiments and transfection studies using Thr308 mutated Akt1 proteins demonstrated that phosphorylation of Akt1 at Thr308 appeared to selectively exclude the active kinase from the nucleus and direct the kinase to the cytoplasmic compartment in keratinocytes upon insulin stimulation. In summary, our data show that phosphorylation of Thr308 during insulin-mediated Akt1 activation is an essential prerequisite to exclude Akt1 from the nuclear compartment.

• OSTI ID: 21143770
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 372; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CULTURES
CELL NUCLEI
HEALING
INSULIN
MICE
MUTATIONS
PHOSPHORYLATION
STIMULATION
WOUNDS