The effect of charged lipids on bacteriorhodopsin membrane reconstitution and its photochemical activities
- College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240 (China)
- School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240 (China)
Bacteriorhodopsin (BR) was reconstituted into artificial lipid membrane containing various charged lipid compositions. The proton pumping activity of BR under flash and continuous illumination, proton permeability across membrane, as well as the decay kinetics of the photocycle intermediate M{sub 412} were studied. The results showed that lipid charges would significantly affect the orientation of BR inserted into lipid membranes. In liposomes containing anionic lipids, BRs were more likely to take natural orientation as in living cells. In neutral or positively charged liposomes, most BRs were reversely assembled, assuming an inside out orientation. Moreover, the lipids charges also affect BR's M intermediate kinetics, especially the slow component in M intermediate decay. The half-life M{sub 412s} increased significantly in BRs in liposomes containing cationic lipids, while decreased in those in anionic liposomes.
- OSTI ID:
- 21143758
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 371, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.04.141; PII: S0006-291X(08)00855-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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