Immune-suppressive activity of punicalagin via inhibition of NFAT activation
- Institute of Traditional Medicine and Bioscience, Daejeon University, Yongun-dong, Dong-gu, Daejeon 300-716 (Korea, Republic of)
- Department of Applied Chemistry, Daejeon University, Yongun-dong, Dong-gu, Daejeon 300-716 (Korea, Republic of)
- Department of Microbiology and Biotechnology, Daejeon University, Yongun-dong, Dong-gu, Daejeon 300-716 (Korea, Republic of)
Since T cell activation is central to the development of autoimmune diseases, we screened a natural product library comprising 1400 samples of medicinal herbal extracts, to identify compounds that suppress T cell activity. Punicalagin (PCG) isolated from the fruit of Punica granatum was identified as a potent immune suppressant, based on its inhibitory action on the activation of the nuclear factor of activated T cells (NFAT). PCG downregulated the mRNA and soluble protein expression of interleukin-2 from anti-CD3/anti-CD28-stimulated murine splenic CD4+ T cells and suppressed mixed leukocytes reaction (MLR) without exhibiting cytotoxicity to the cells. In vivo, the PCG treatment inhibited phorbol 12-myristate 13-acetate (PMA)-induced chronic ear edema in mice and decreased CD3+ T cell infiltration of the inflamed tissue. These results suggest that PCG could be a potential candidate for the therapeutics of various immune pathologies.
- OSTI ID:
- 21143756
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 371; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
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